Anti-Myosin light chain kinase/MLCK (phospho S1760) antibody (ab200809)
- Datasheet
- References (2)
- Protocols
Overview
-
Product name
Anti-Myosin light chain kinase/MLCK (phospho S1760) antibody
See all Myosin light chain kinase/MLCK primary antibodies -
Description
Rabbit polyclonal to Myosin light chain kinase/MLCK (phospho S1760) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rabbit, Cow
Predicted to work with: Human
-
Immunogen
Synthetic peptide corresponding to Human Myosin light chain kinase/MLCK (phospho S1760). A phosphopeptide derived from the region of Human Myosin light chain kinase/ MLCK that contains serine 1760.
Database link: Q15746 -
Positive control
- KCl-treated rabbit muscle extracts; COS cells over-expressing rabbit smooth muscle short Myosin light chain kinase/MLCK, phosphorylated in vitro by PKA.
-
General notes
Previously labelled as Myosin light chain kinase.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab200809 has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Myosin light chain kinase/MLCK. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab200809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 211 kDa. |
Target
-
Function
Calcium/calmodulin-dependent enzyme implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activty (By similarity). Implicated in the regulation of endothelial as well as vascular permeability. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis. -
Tissue specificity
Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 fibronectin type-III domain.
Contains 9 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsMLCK is probably down-regulated by phosphorylation.
The C-terminus is deglutamylated by AGTPBP1/ CCP1, AGBL1/CCP4 and AGBL4/CCP6, leading to the formation of Myosin light chain kinase, smooth muscle, deglutamylated form. The consequences of C-terminal deglutamylation are unknown. - Information by UniProt
-
Database links
- Entrez Gene: 338037 Cow
- Entrez Gene: 4638 Human
- Entrez Gene: 107589 Mouse
- Entrez Gene: 100009143 Rabbit
- Omim: 600922 Human
- SwissProt: Q28824 Cow
- SwissProt: Q15746 Human
- SwissProt: Q6PDN3 Mouse
see all -
Alternative names
- deglutamylated form antibody
- DKFZp686I10125 antibody
- EC 2.7.11.18 antibody
see all
Images
-
Western blot - Anti-Myosin light chain kinase/MLCK (phospho S1760) antibody (ab200809)All lanes : Anti-Myosin light chain kinase/MLCK (phospho S1760) antibody (ab200809) at 1/1000 dilution
Lane 1 : Extracts of COS cells transfected with rabbit muscle Myosin light chain kinase/MLCK, unstimulated
Lane 2 : Extracts of COS cells transfected with rabbit muscle Myosin light chain kinase/MLCK phosphorylated in vitro by 0.05 µg of PKA
Lane 3 : Extracts of COS cells transfected with rabbit muscle Myosin light chain kinase/MLCK phosphorylated in vitro by 0.05 µg of PKA with the non-phosphopeptide corresponding to the phosphopeptide immunogen
Lane 4 : Extracts of COS cells transfected with rabbit muscle Myosin light chain kinase/MLCK phosphorylated in vitro by 0.05 µg of PKA with a generic phosphoserine-containing peptide
Lane 5 : Extracts of COS cells transfected with rabbit muscle Myosin light chain kinase/MLCK phosphorylated in vitro by 0.05 µg of PKA with the phosphopeptide immunogen
Lane 6 : Extracts of COS cells transfected with rabbit muscle Myosin light chain kinase/MLCK phosphorylated in vitro by 0.05 µg of PKA, treated with
lambda phophatase
Secondary
All lanes : goat F(ab’)2 anti-rabbit IgG HRP
Predicted band size: 211 kDaResolved by SDS-PAGE on a 4-12% Tris-glycine gel and transferred to PVDF. The membrane was left untreated (Lanes 1-5) or treated with lambda phophatase (Lane 6), blocked with a 5% BSA-TBST buffer for one hour at room temperature and then incubated with ab200809 for two hours at room temperature in 3% BSA-TBST buffer.
N.B. 0.05 μg of PKA (per μg of extract) for 10 minutes at 37 ºC.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Cheng YC et al. Magnolol Inhibits Human Glioblastoma Cell Migration by Regulating N-Cadherin. J Neuropathol Exp Neurol 77:426-436 (2018). Read more (PubMed: 29788114) »
- Boire A et al. Complement Component 3 Adapts the Cerebrospinal Fluid for Leptomeningeal Metastasis. Cell 168:1101-1113.e13 (2017). Read more (PubMed: 28283064) »
