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Signal Transduction Cytoskeleton / ECM Cytoskeleton Motor Proteins Myosin
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Anti-Myosin light chain kinase/MLCK (phospho S1760) antibody (ab200809)

  • Datasheet
Reviews (1) Submit a question References (2)

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Western blot - Anti-Myosin light chain kinase/MLCK (phospho S1760) antibody (ab200809)

    Key features and details

    • Rabbit polyclonal to Myosin light chain kinase/MLCK (phospho S1760)
    • Suitable for: WB
    • Reacts with: Mouse, Rabbit, Cow
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
    Product image
    Recombinant human Myosin light chain kinase/MLCK protein (ab55674)

    View more associated products

    Overview

    • Product name

      Anti-Myosin light chain kinase/MLCK (phospho S1760) antibody
      See all Myosin light chain kinase/MLCK primary antibodies
    • Description

      Rabbit polyclonal to Myosin light chain kinase/MLCK (phospho S1760)
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Rabbit, Cow
      Predicted to work with: Human
    • Immunogen

      Synthetic peptide corresponding to Human Myosin light chain kinase/MLCK (phospho S1760). A phosphopeptide derived from the region of Human Myosin light chain kinase/ MLCK that contains serine 1760.
      Database link: Q15746

    • Positive control

      • KCl-treated rabbit muscle extracts; COS cells over-expressing rabbit smooth muscle short Myosin light chain kinase/MLCK, phosphorylated in vitro by PKA.
    • General notes

      Previously labelled as Myosin light chain kinase.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.30
      Preservative: 0.05% Sodium azide
      Constituents: 50% Glycerol, 0.1% BSA, 49% PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab200809 has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Myosin light chain kinase/MLCK.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Motor Proteins
      • Myosin

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human Myosin light chain kinase/MLCK protein (ab55674)

    Applications

    Our Abpromise guarantee covers the use of ab200809 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/1000. Predicted molecular weight: 211 kDa.

    Target

    • Function

      Calcium/calmodulin-dependent enzyme implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activty (By similarity). Implicated in the regulation of endothelial as well as vascular permeability. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis.
    • Tissue specificity

      Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues.
    • Sequence similarities

      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
      Contains 1 fibronectin type-III domain.
      Contains 9 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      MLCK is probably down-regulated by phosphorylation.
      The C-terminus is deglutamylated by AGTPBP1/ CCP1, AGBL1/CCP4 and AGBL4/CCP6, leading to the formation of Myosin light chain kinase, smooth muscle, deglutamylated form. The consequences of C-terminal deglutamylation are unknown.
    • Target information above from: UniProt accession Q15746 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 338037 Cow
      • Entrez Gene: 4638 Human
      • Entrez Gene: 107589 Mouse
      • Entrez Gene: 100009143 Rabbit
      • Omim: 600922 Human
      • SwissProt: Q28824 Cow
      • SwissProt: Q15746 Human
      • SwissProt: Q6PDN3 Mouse
      • SwissProt: P29294 Rabbit
      • Unigene: 477375 Human
      • Unigene: 33360 Mouse
      see all
    • Alternative names

      • deglutamylated form antibody
      • DKFZp686I10125 antibody
      • EC 2.7.11.18 antibody
      • FLJ12216 antibody
      • Kinase related protein antibody
      • Kinase-related protein antibody
      • KRP antibody
      • MLCK antibody
      • MLCK1 antibody
      • MLCK108 antibody
      • MLCK210 antibody
      • MSTP083 antibody
      • MYLK antibody
      • MYLK_HUMAN antibody
      • MYLK1 antibody
      • Myosin light chain kinase antibody
      • Myosin light polypeptide kinase antibody
      • OTTHUMP00000180642 antibody
      • OTTHUMP00000180643 antibody
      • smMLCK antibody
      • smooth muscle antibody
      • Smooth muscle myosin light chain kinase antibody
      • Telokin antibody
      see all

    Images

    • Western blot - Anti-Myosin light chain kinase/MLCK (phospho S1760) antibody (ab200809)
      Western blot - Anti-Myosin light chain kinase/MLCK (phospho S1760) antibody (ab200809)
      All lanes : Anti-Myosin light chain kinase/MLCK (phospho S1760) antibody (ab200809) at 1/1000 dilution

      Lane 1 : Extracts of COS cells transfected with rabbit muscle Myosin light chain kinase/MLCK, unstimulated
      Lane 2 : Extracts of COS cells transfected with rabbit muscle Myosin light chain kinase/MLCK phosphorylated in vitro by 0.05 µg of PKA
      Lane 3 : Extracts of COS cells transfected with rabbit muscle Myosin light chain kinase/MLCK phosphorylated in vitro by 0.05 µg of PKA with the non-phosphopeptide corresponding to the phosphopeptide immunogen
      Lane 4 : Extracts of COS cells transfected with rabbit muscle Myosin light chain kinase/MLCK phosphorylated in vitro by 0.05 µg of PKA with a generic phosphoserine-containing peptide
      Lane 5 : Extracts of COS cells transfected with rabbit muscle Myosin light chain kinase/MLCK phosphorylated in vitro by 0.05 µg of PKA with the phosphopeptide immunogen
      Lane 6 : Extracts of COS cells transfected with rabbit muscle Myosin light chain kinase/MLCK phosphorylated in vitro by 0.05 µg of PKA, treated with
      lambda phophatase

      Secondary
      All lanes : goat F(ab’)2 anti-rabbit IgG HRP

      Predicted band size: 211 kDa



      Resolved by SDS-PAGE on a 4-12% Tris-glycine gel and transferred to PVDF. The membrane was left untreated (Lanes 1-5) or treated with lambda phophatase (Lane 6), blocked with a 5% BSA-TBST buffer for one hour at room temperature and then incubated with ab200809 for two hours at room temperature in 3% BSA-TBST buffer.

       

      N.B. 0.05 μg of PKA (per μg of extract) for 10 minutes at 37 ºC.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (2)

    Publishing research using ab200809? Please let us know so that we can cite the reference in this datasheet.

    ab200809 has been referenced in 2 publications.

    • Cheng YC  et al. Magnolol Inhibits Human Glioblastoma Cell Migration by Regulating N-Cadherin. J Neuropathol Exp Neurol 77:426-436 (2018). PubMed: 29788114
    • Boire A  et al. Complement Component 3 Adapts the Cerebrospinal Fluid for Leptomeningeal Metastasis. Cell 168:1101-1113.e13 (2017). PubMed: 28283064

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-Myosin light chain kinase/MLCK (phospho S1760) antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (airway smooth muscle cell)
    Gel Running Conditions
    Reduced Denaturing (12%gel)
    Loading amount
    60 µg
    Specification
    airway smooth muscle cell
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Mrs. Xiao Jie Han

    Verified customer

    Submitted Aug 17 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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