Overview

  • Product name
    Anti-Myosin light chain (phospho S1) antibody
    See all Myosin light chain primary antibodies
  • Description
    Rabbit polyclonal to Myosin light chain (phospho S1)
  • Host species
    Rabbit
  • Specificity
    The immunogen sequence is highly conserved in Human, rat and mouse MYL9, and in other smooth muscle and non-muscle Myosin light chains.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Cow Myosin light chain (N terminal) conjugated to keyhole limpet haemocyanin.

  • Positive control
    • A7r5 cells; EGF or calyculin A treated Human A431 cell lysates; Mouse C2C12, rat PC12, and rabbit fibroblasts treated with calyculin A.

Properties

Applications

Our Abpromise guarantee covers the use of ab157747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 20 kDa.
ICC/IF 1/100.

Target

  • Function
    Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
  • Tissue specificity
    Smooth muscle tissues and in some, but not all, nonmuscle cells.
  • Sequence similarities
    Contains 3 EF-hand domains.
  • Post-translational
    modifications
    Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
  • Information by UniProt
  • Database links
  • Alternative names
    • 20 kDa myosin light chain antibody
    • KMLC antibody
    • LC20 antibody
    • MLC-2C antibody
    • MLCK2 antibody
    • MRLC1 antibody
    • MYL9 antibody
    • MYL9_HUMAN antibody
    • Myosin regulatory light chain 2 antibody
    • Myosin regulatory light chain 2, smooth muscle isoform antibody
    • Myosin regulatory light chain 9 antibody
    • Myosin regulatory light chain MRLC1 antibody
    • Myosin regulatory light polypeptide 9 antibody
    • Myosin RLC antibody
    • Myosin, light chain 9, regulatory antibody
    • MYRL2 antibody
    • smooth muscle isoform antibody
    see all

Images

  • Immunofluorescent analysis of paraformaldehyde-fixed A7r5 cells labeling Myosin light chain with ab157747 at 1/100 dilution (left). Cells were dual stained with mouse anti-Myosin light chain antibody (middle panel) and the overlay of staining patterns is shown to the right. Goat anti-mouse Dylight 488 and goat anti-rabbit Dylight 594 were used for detection of primary antibodies.
  • All lanes : Anti-Myosin light chain (phospho S1) antibody (ab157747)

    Lane 1 : C2C12 cell lysate, untreated
    Lane 2 : C2C12 cell lysate treated
    with Lambda phosphatase

    Predicted band size: 20 kDa

References

ab157747 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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