For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    myosin-light-chain-phospho-s20-antibody-ab2480.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Cytoskeleton / ECM Cytoskeleton Motor Proteins Myosin
Share by email

Anti-Myosin light chain (phospho S20) antibody (ab2480)

  • Datasheet
Reviews (15)Q&A (16)References (116)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Myosin light chain (phospho S20) antibody (ab2480)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain (phospho S20) antibody (ab2480)
  • Western blot - Anti-Myosin light chain (phospho S20) antibody (ab2480)

Key features and details

  • Rabbit polyclonal to Myosin light chain (phospho S20)
  • Suitable for: ELISA, ICC/IF, IHC-FoFr, WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human, Caenorhabditis elegans, Recombinant fragment
  • Isotype: IgG

You may also be interested in

Primary
Product image
Anti-AKT1 (phospho S473) antibody [EP2109Y] - BSA and Azide free (ab215873)
Lysate
C2C12 whole cell lysate (ab7182)
Primary
Product image
Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594)

View more associated products

Overview

  • Product name

    Anti-Myosin light chain (phospho S20) antibody
    See all Myosin light chain primary antibodies
  • Description

    Rabbit polyclonal to Myosin light chain (phospho S20)
  • Host species

    Rabbit
  • Specificity

    This phospho specific polyclonal antibody is specific for phosphorylated pS20 of human myosin light chain. Reactivity with non-phosphorylated human myosin light chain is less than 1% by ELISA. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.

    Customers have seen variable performance with Xenopus laevis.

  • Tested applications

    Suitable for: ELISA, ICC/IF, IHC-FoFr, WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Caenorhabditis elegans, Recombinant fragment
    Predicted to work with: Bird, Mammals
  • Immunogen

    Synthetic peptide corresponding to Human Myosin light chain aa 1-100 (phospho S20) conjugated to keyhole limpet haemocyanin.
    Database link: J3QRS3

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Myosin
    • Stem Cells
    • Mesenchymal Stem Cells
    • Myogenesis
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Myosins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
ICC/IF (5)
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 19279134

Trichloroacetic acid was used for tissue fixation.

WB (7)
1/500 - 1/2000. Predicted molecular weight: 20 kDa.

We recommend using BSA for blocking when using phospho-specific antibodies.

IHC-P (2)
Use a concentration of 2.5 µg/ml.
IP
Use at an assay dependent concentration.
Notes
ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 19279134

Trichloroacetic acid was used for tissue fixation.

WB
1/500 - 1/2000. Predicted molecular weight: 20 kDa.

We recommend using BSA for blocking when using phospho-specific antibodies.

IHC-P
Use a concentration of 2.5 µg/ml.
IP
Use at an assay dependent concentration.

Target

  • Function

    Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
  • Tissue specificity

    Smooth muscle tissues and in some, but not all, nonmuscle cells.
  • Sequence similarities

    Contains 3 EF-hand domains.
  • Post-translational
    modifications

    Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
  • Target information above from: UniProt accession P24844 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10398 Human
    • Entrez Gene: 98932 Mouse
    • Entrez Gene: 296313 Rat
    • Omim: 609905 Human
    • SwissProt: P24844 Human
    • SwissProt: Q9CQ19 Mouse
    • SwissProt: Q64122 Rat
    • Unigene: 504687 Human
    • Unigene: 271770 Mouse
    • Unigene: 228729 Rat
    • Unigene: 6870 Rat
    see all
  • Alternative names

    • 20 kDa myosin light chain antibody
    • KMLC antibody
    • LC20 antibody
    • MLC-2C antibody
    • MLCK2 antibody
    • MRLC1 antibody
    • MYL9 antibody
    • MYL9_HUMAN antibody
    • Myosin regulatory light chain 2 antibody
    • Myosin regulatory light chain 2, smooth muscle isoform antibody
    • Myosin regulatory light chain 9 antibody
    • Myosin regulatory light chain MRLC1 antibody
    • Myosin regulatory light polypeptide 9 antibody
    • Myosin RLC antibody
    • Myosin, light chain 9, regulatory antibody
    • MYRL2 antibody
    • smooth muscle isoform antibody
    see all

Images

  • Western blot - Anti-Myosin light chain (phospho S20) antibody (ab2480)
    Western blot - Anti-Myosin light chain (phospho S20) antibody (ab2480)
    All lanes : Anti-Myosin light chain (phospho S20) antibody (ab2480) at 1/1000 dilution

    Lane 1 : Regulatory Light Chain Non-Phospho recombinant protein, 50 ng
    Lane 2 : Regulatory Light Chain Phospho recombinant protein, 50 ng
    Lane 3 : Smooth Muscle Non-Phospho recombinant protein, 50 ng
    Lane 4 : Smooth Muscle Phospho recombinant protein, 50 ng

    Secondary
    All lanes : Peroxidase rabbit secondary antibody at 1/40000 dilution

    Predicted band size: 20 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain (phospho S20) antibody (ab2480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain (phospho S20) antibody (ab2480)

    IHC-P of ab2480 at 2.5 µg/ml staining both vascular and myometrial smooth muscle cells of the human uterus. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

  • Western blot - Anti-Myosin light chain (phospho S20) antibody (ab2480)
    Western blot - Anti-Myosin light chain (phospho S20) antibody (ab2480)

    Rabbit polyclonal to phospho Myosin Light Chain phospho Myosin Light Chain (Ser 20) (ab2480) used at a 1/5000 dilution to detect myosin light chain by Western blot. Either 13 ug (lanes 1-3) or 20 ug (lanes 4-7) of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE.  Samples were either mock-treated or CLA treated:
    Lane 1 : untreated 45 min
    Lane 2 : CLA 50 nm 45 min
    Lane 3 : CLA 100 nm 45 min
    Lane 4 : A21 untreated 45 min
    Lane 5 : A22 A23187 5 min
    Lane 6 : A23 A23187 15 min
    Lane 7 : A24 A23187 60 min
    After washing, a 1/5,000 dilution of anti-rabbit HRP (ab7090) was used as secondary.

    Rabbit polyclonal to phospho Myosin Light Chain phospho Myosin Light Chain (Ser 20) (ab2480) used at a 1/5000 dilution to detect myosin light chain by Western blot. Either 13 ug (lanes 1-3) or 20 ug (lanes 4-7) of a mouse cardiac myocyte lysate was loaded on a 4-20

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (116)

Publishing research using ab2480? Please let us know so that we can cite the reference in this datasheet.

ab2480 has been referenced in 116 publications.

  • Li B  et al. Iron deficiency promotes aortic medial degeneration via destructing cytoskeleton of vascular smooth muscle cells. Clin Transl Med 11:e276 (2021). PubMed: 33463069
  • Winter MR  et al. Calcium-vesicles perform active diffusion in the sea urchin embryo during larval biomineralization. PLoS Comput Biol 17:e1008780 (2021). PubMed: 33617532
  • Gong W  et al. CCL4-mediated targeting of spleen tyrosine kinase (Syk) inhibitor using nanoparticles alleviates inflammatory bowel disease. Clin Transl Med 11:e339 (2021). PubMed: 33634985
  • Hatte G  et al. Adherens junctions are involved in polarized contractile ring formation in dividing epithelial cells of Xenopus laevis embryos. Exp Cell Res 402:112525 (2021). PubMed: 33662366
  • Young RE  et al. Smooth Muscle Differentiation Is Essential for Airway Size, Tracheal Cartilage Segmentation, but Dispensable for Epithelial Branching. Dev Cell 53:73-85.e5 (2020). PubMed: 32142630
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 31 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin light chain (phospho S20) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U87-MG)
Permeabilization
Yes - 0.5% triton
Specification
U87-MG
Blocking step
Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Dec 02 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin light chain (phospho S20) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (MDCK)
Permeabilization
Yes - 0.2% Triton X100
Specification
MDCK
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Mar 11 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myosin light chain (phospho S20) antibody

Good
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (head)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA based buffer pH 7.8
Permeabilization
No
Specification
head
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Sep 13 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myosin light chain (phospho S20) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Prostate)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate, pH 6
Permeabilization
No
Specification
Prostate
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Nov 28 2017

IHC - Wholemount abreview for Anti-Myosin light chain (phospho S20) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Hydra vulgaris Tissue (morphogenesis)
Specification
morphogenesis
Read More

Mr. Oliver Holz

Verified customer

Submitted Jul 21 2017

Western blot abreview for Anti-Myosin light chain (phospho S20) antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% BIS TRIS)
Loading amount
15 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Sep 08 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin light chain (phospho S20) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human arterial myocytes)
Specification
Human arterial myocytes
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Apr 08 2015

Western blot

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15)
Sample
Human Tissue lysate - whole (Heart)
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted May 22 2013

Question

Do you have any myosin light chain antibodies that detect both the phosphorylated and non-phosphorylated bands on a western blot? I have ab11082 which someone told me is able to detect both bands, but I have had no success so far. The best I can do is blot with ab11082 and then with ab2480 to get the phosphorylated band, but I am wanting to compare the bands and would prefer just one antibody for accuracy.

Read More

Abcam community

Verified customer

Asked on Dec 20 2012

Answer

Thank you for contacting us.

Are you looking for a specific light chain (i.e. 2 or 3)? We only have 2 other anti-myosin light chain antibodies as ab97891 and ab88726 but we don't have any WB images showing 2 bands for phopsho and unphosphorylated protein. You may have to do a WB with a phospho-specific antibody if you want to see clear distinctions between phosphorylated and total proteins.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Dec 20 2012

Question

Does that mean that Abcam didn't test the
cross reactivity of this antibody to skeletal muscle isoform of
phosphorylated myosin regulatory light chain? Could you also let me know by
what means you found that this antibody doesn't cross react with cardiac isoform?

Read More

Abcam community

Verified customer

Asked on Dec 06 2012

Answer



You are correct that Abcam has not specifically tested cross reactivity of ab2480 with skeletal muscle isoform MLC. This cross reactivity is predicted based on similarity of the immunogen sequence to skeletal muscle MLC. Likewise, ab2480 has not been tested for reactivity to cardiac MLC but the immunogen sequence does not exist in the cardiac MLC.

Read More

Abcam Scientific Support

Answered on Dec 06 2012

1-10 of 31 Abreviews or Q&A

  •  Previous
  • 1
  • 2
  • 3
  • 4
  • Next 

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.