Anti-Myosin light chain (phospho S20) antibody (ab2480)
Key features and details
- Rabbit polyclonal to Myosin light chain (phospho S20)
- Suitable for: ELISA, ICC/IF, IHC-FoFr, WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human, Caenorhabditis elegans, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Myosin light chain (phospho S20) antibody
See all Myosin light chain primary antibodies -
Description
Rabbit polyclonal to Myosin light chain (phospho S20) -
Host species
Rabbit -
Specificity
This phospho specific polyclonal antibody is specific for phosphorylated pS20 of human myosin light chain. Reactivity with non-phosphorylated human myosin light chain is less than 1% by ELISA. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.
Customers have seen variable performance with Xenopus laevis.
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Tested applications
Suitable for: ELISA, ICC/IF, IHC-FoFr, WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Caenorhabditis elegans, Recombinant fragment
Predicted to work with: Bird, Mammals
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Immunogen
Synthetic peptide corresponding to Human Myosin light chain aa 1-100 (phospho S20) conjugated to keyhole limpet haemocyanin.
Database link: J3QRS3 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| ICC/IF | (5) |
Use at an assay dependent concentration.
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| IHC-FoFr |
Use at an assay dependent concentration. PubMed: 19279134
Trichloroacetic acid was used for tissue fixation. |
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| WB | (7) |
1/500 - 1/2000. Predicted molecular weight: 20 kDa.
We recommend using BSA for blocking when using phospho-specific antibodies. |
| IHC-P | (2) |
Use a concentration of 2.5 µg/ml.
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| IP |
Use at an assay dependent concentration.
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| Notes |
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-FoFr
Use at an assay dependent concentration. PubMed: 19279134 Trichloroacetic acid was used for tissue fixation. |
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WB
1/500 - 1/2000. Predicted molecular weight: 20 kDa. We recommend using BSA for blocking when using phospho-specific antibodies. |
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IHC-P
Use a concentration of 2.5 µg/ml. |
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IP
Use at an assay dependent concentration. |
Target
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Function
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. -
Tissue specificity
Smooth muscle tissues and in some, but not all, nonmuscle cells. -
Sequence similarities
Contains 3 EF-hand domains. -
Post-translational
modificationsPhosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. - Information by UniProt
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Database links
- Entrez Gene: 10398 Human
- Entrez Gene: 98932 Mouse
- Entrez Gene: 296313 Rat
- Omim: 609905 Human
- SwissProt: P24844 Human
- SwissProt: Q9CQ19 Mouse
- SwissProt: Q64122 Rat
- Unigene: 504687 Human
see all -
Alternative names
- 20 kDa myosin light chain antibody
- KMLC antibody
- LC20 antibody
see all
Images
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Western blot - Anti-Myosin light chain (phospho S20) antibody (ab2480)All lanes : Anti-Myosin light chain (phospho S20) antibody (ab2480) at 1/1000 dilution
Lane 1 : Regulatory Light Chain Non-Phospho recombinant protein, 50 ng
Lane 2 : Regulatory Light Chain Phospho recombinant protein, 50 ng
Lane 3 : Smooth Muscle Non-Phospho recombinant protein, 50 ng
Lane 4 : Smooth Muscle Phospho recombinant protein, 50 ng
Secondary
All lanes : Peroxidase rabbit secondary antibody at 1/40000 dilution
Predicted band size: 20 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain (phospho S20) antibody (ab2480)IHC-P of ab2480 at 2.5 µg/ml staining both vascular and myometrial smooth muscle cells of the human uterus. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
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Western blot - Anti-Myosin light chain (phospho S20) antibody (ab2480)Rabbit polyclonal to phospho Myosin Light Chain phospho Myosin Light Chain (Ser 20) (ab2480) used at a 1/5000 dilution to detect myosin light chain by Western blot. Either 13 ug (lanes 1-3) or 20 ug (lanes 4-7) of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock-treated or CLA treated:
Lane 1 : untreated 45 min
Lane 2 : CLA 50 nm 45 min
Lane 3 : CLA 100 nm 45 min
Lane 4 : A21 untreated 45 min
Lane 5 : A22 A23187 5 min
Lane 6 : A23 A23187 15 min
Lane 7 : A24 A23187 60 min
After washing, a 1/5,000 dilution of anti-rabbit HRP (ab7090) was used as secondary.Rabbit polyclonal to phospho Myosin Light Chain phospho Myosin Light Chain (Ser 20) (ab2480) used at a 1/5000 dilution to detect myosin light chain by Western blot. Either 13 ug (lanes 1-3) or 20 ug (lanes 4-7) of a mouse cardiac myocyte lysate was loaded on a 4-20
Protocols
Datasheets and documents
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Datasheet download
References (116)
ab2480 has been referenced in 116 publications.
- Li B et al. Iron deficiency promotes aortic medial degeneration via destructing cytoskeleton of vascular smooth muscle cells. Clin Transl Med 11:e276 (2021). PubMed: 33463069
- Winter MR et al. Calcium-vesicles perform active diffusion in the sea urchin embryo during larval biomineralization. PLoS Comput Biol 17:e1008780 (2021). PubMed: 33617532
- Gong W et al. CCL4-mediated targeting of spleen tyrosine kinase (Syk) inhibitor using nanoparticles alleviates inflammatory bowel disease. Clin Transl Med 11:e339 (2021). PubMed: 33634985
- Hatte G et al. Adherens junctions are involved in polarized contractile ring formation in dividing epithelial cells of Xenopus laevis embryos. Exp Cell Res 402:112525 (2021). PubMed: 33662366
- Young RE et al. Smooth Muscle Differentiation Is Essential for Airway Size, Tracheal Cartilage Segmentation, but Dispensable for Epithelial Branching. Dev Cell 53:73-85.e5 (2020). PubMed: 32142630
