Western blot abreview for Anti-Myosin Phosphatase 1+Myosin Phosphatase 2 antibody [YE336]

Good
Abreviews
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (7.5%)
Sample
Rabbit Cell lysate - whole cell (NSCLC)
Specification
NSCLC
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk/TBST
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Peroxidase-AffiniPure Goat Anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Bands
Specific: 110 kDa Non-specific: 60 kDa
Exposure
30 second(s)
Negative control
scrambled siRNA
Positive control
siRNA targeting MYPT1

Additional data

Additional Notes
lane 1 - ladder lane 2 - control siRNA (scrambled) lane 3 - MYPT1 siRNA bottom row of bands is GAPDH, which was used as an internal control.

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Submitted Sep 27 2013

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