Overview

  • Product name
    Anti-Myosin Phosphatase 2 antibody
  • Description
    Mouse polyclonal to Myosin Phosphatase 2
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Full length protein, corresponding to amino acids 1-386 of Human Myosin Phosphatase 2 (AAH34430).

  • Positive control
    • Myosin Phosphatase 2 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab168988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.

Target

  • Relevance
    Myosin light chain phosphatase (MLCP) consists of three subunits- catalytic subunit, large subunit/myosin binding subunit (MBS) and small subunit (sm-M20). This gene is a multi-functional gene which encodes both MBS and sm-M20. MLCP regulates myosins and the dephosphorylation is enhanced by the presence of MBS. The sm-M20 is suggested to play a regulatory role in muscle contraction by binding to MBS. MBS is also encoded by another gene, myosin light chain phosphatase target subunit 1. sm-M20 shows higher binding affinity to this gene product than to myosin light chain phosphatase target subunit 2-MBS even though the two MBS proteins are highly similar. Although both MBSs increase the activity of MLCP, myosin light chain phosphatase target subunit 1-MBS is a more efficient activator. There are four alternatively spliced transcript variants described; two alter the MBS coding region and two alter the sm-M20 coding region of this gene.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • Myosin Phosphatase 2 antibody
    • Myosin phosphatase regulatory subunit antibody
    • Myosin phosphatase targeting subunit 2 antibody
    • Myosin phosphatase, target subunit 2 antibody
    • MYPT2 antibody
    • PP1bp55 antibody
    • PPP1R12B antibody
    • Protein phosphatase 1 regulatory subunit 12B antibody
    • Protein phosphatase 1, regulatory (inhibitor) subunit 12B antibody
    see all

Images

  • All lanes : Anti-Myosin Phosphatase 2 antibody (ab168988) at 1 µg/ml

    Lane 1 : Myosin Phosphatase 2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 43 kDa

References

ab168988 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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