Overview

  • Product name

    Anti-Myosin Phosphatase antibody
    See all Myosin Phosphatase primary antibodies
  • Description

    Rabbit polyclonal to Myosin Phosphatase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Myosin Phosphatase aa 179-481. The exact sequence is proprietary.
    Database link: O14974

  • Positive control

    • Human rhabdomyosarcoma RD cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab184121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Regulates myosin phosphatase activity.
  • Tissue specificity

    Expressed in striated muscles, specifically in type 2a fibers (at protein level).
  • Sequence similarities

    Contains 6 ANK repeats.
  • Developmental stage

    Induced by 2-fold during pregnancy, including in abdominus rectus muscle.
  • Domain

    Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.
  • Post-translational
    modifications

    Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
    In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.
  • Cellular localization

    Cytoplasm. Along actomyosin filaments and stress fibers.
  • Information by UniProt
  • Database links

  • Alternative names

    • M130 antibody
    • MBS antibody
    • Myosin binding subunit antibody
    • Myosin phosphatase target subunit 1 antibody
    • Myosin phosphatase targeting subunit 1 antibody
    • Myosin phosphatase-targeting subunit 1 antibody
    • MYPT1 antibody
    • MYPT1_HUMAN antibody
    • PPP1R12A antibody
    • Protein phosphatase 1 regulatory inhibitor subunit 12A antibody
    • Protein phosphatase 1 regulatory subunit 12A antibody
    • Protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody
    • Protein phosphatase myosin binding subunit antibody
    • Protein phosphatase myosin-binding subunit antibody
    see all

Images

  • Immunofluorescence analysis of methanol fixed Human RD cells, staining Myosin Phosphatase (green) with ab184121 at 1/1000 dilution. Nuclei were stained with Hoechst 33342 (blue).

References

ab184121 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab184121.
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