Key features and details
- Rabbit polyclonal to Myosin Phosphatase
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Myosin Phosphatase antibody
See all Myosin Phosphatase primary antibodies
DescriptionRabbit polyclonal to Myosin Phosphatase
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Rhesus monkey
Recombinant fragment within Human Myosin Phosphatase aa 179-481. The exact sequence is proprietary.
Database link: O14974
- Human rhabdomyosarcoma RD cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab184121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
FunctionRegulates myosin phosphatase activity.
Tissue specificityExpressed in striated muscles, specifically in type 2a fibers (at protein level).
Sequence similaritiesContains 6 ANK repeats.
Developmental stageInduced by 2-fold during pregnancy, including in abdominus rectus muscle.
DomainHeterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.
modificationsPhosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.
Cellular localizationCytoplasm. Along actomyosin filaments and stress fibers.
- Information by UniProt
- M130 antibody
- MBS antibody
- Myosin binding subunit antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab184121 has not yet been referenced specifically in any publications.