• Product name

    Anti-Myosin Phosphatase antibody
    See all Myosin Phosphatase primary antibodies
  • Description

    Rabbit polyclonal to Myosin Phosphatase
  • Host species

  • Tested applications

    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human Myosin Phosphatase around the phosphorylation site of threonine 853 (R-S-TP-G-V).

  • Positive control

    • Human brain tissue and NIH/3T3 cell extracts.



Our Abpromise guarantee covers the use of ab59235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 115 kDa).


  • Function

    Regulates myosin phosphatase activity.
  • Tissue specificity

    Expressed in striated muscles, specifically in type 2a fibers (at protein level).
  • Sequence similarities

    Contains 6 ANK repeats.
  • Developmental stage

    Induced by 2-fold during pregnancy, including in abdominus rectus muscle.
  • Domain

    Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.
  • Post-translational

    Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
    In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.
  • Cellular localization

    Cytoplasm. Along actomyosin filaments and stress fibers.
  • Information by UniProt
  • Database links

  • Alternative names

    • M130 antibody
    • MBS antibody
    • Myosin binding subunit antibody
    • Myosin phosphatase target subunit 1 antibody
    • Myosin phosphatase targeting subunit 1 antibody
    • Myosin phosphatase-targeting subunit 1 antibody
    • MYPT1 antibody
    • MYPT1_HUMAN antibody
    • PPP1R12A antibody
    • Protein phosphatase 1 regulatory inhibitor subunit 12A antibody
    • Protein phosphatase 1 regulatory subunit 12A antibody
    • Protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody
    • Protein phosphatase myosin binding subunit antibody
    • Protein phosphatase myosin-binding subunit antibody
    see all


  • All lanes : Anti-Myosin Phosphatase antibody (ab59235) at 1/500 dilution

    Lane 1 : NIH3T3 cell extract
    Lane 2 : NIH3T3 cell extract with immunising peptide

    Predicted band size: 115 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?

  • ab59235 at 1/50 dilution staining Myosin Phosphatase in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.


This product has been referenced in:

  • Liang Y  et al. Decreased Expression of MYPT1 Contributes to Tumor Angiogenesis and Poor Patient Prognosis in Human Prostate Cancer. Curr Mol Med 18:100-108 (2018). Read more (PubMed: 29974831) »
  • Wang XQ  et al. Resveratrol enhances vascular reactivity in mice following lipopolysaccharide challenge via the RhoA-ROCK-MLCP pathway. Exp Ther Med 14:308-316 (2017). Read more (PubMed: 28672931) »
See all 5 Publications for this product

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