• Product name
    Anti-Myosin Phosphatase antibody [Y110]
    See all Myosin Phosphatase primary antibodies
  • Description
    Rabbit monoclonal [Y110] to Myosin Phosphatase
  • Host species
  • Specificity
    Ab32393 recognises Myosin Phosphatase. It can detect all four splice isoforms of Myosin Phosphatase.
  • Tested applications
    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Myosin Phosphatase aa 1-100. The exact sequence is proprietary.

  • Positive control
    • 293 cell lysate.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab32393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 125 kDa (predicted molecular weight: 115 kDa).
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC or IP.
  • Target

    • Function
      Regulates myosin phosphatase activity.
    • Tissue specificity
      Expressed in striated muscles, specifically in type 2a fibers (at protein level).
    • Sequence similarities
      Contains 6 ANK repeats.
    • Developmental stage
      Induced by 2-fold during pregnancy, including in abdominus rectus muscle.
    • Domain
      Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.
    • Post-translational
      Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
      In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.
    • Cellular localization
      Cytoplasm. Along actomyosin filaments and stress fibers.
    • Information by UniProt
    • Database links
    • Alternative names
      • M130 antibody
      • MBS antibody
      • Myosin binding subunit antibody
      • Myosin phosphatase target subunit 1 antibody
      • Myosin phosphatase targeting subunit 1 antibody
      • Myosin phosphatase-targeting subunit 1 antibody
      • MYPT1 antibody
      • MYPT1_HUMAN antibody
      • PPP1R12A antibody
      • Protein phosphatase 1 regulatory inhibitor subunit 12A antibody
      • Protein phosphatase 1 regulatory subunit 12A antibody
      • Protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody
      • Protein phosphatase myosin binding subunit antibody
      • Protein phosphatase myosin-binding subunit antibody
      see all



    This product has been referenced in:
    • Thompson JM  et al. Rho-associated kinase 1 inhibition is synthetically lethal with von Hippel-Lindau deficiency in clear cell renal cell carcinoma. Oncogene 36:1080-1089 (2017). Read more (PubMed: 27841867) »
    • Petrakova OS  et al. Comparative analysis reveals similarities between cultured submandibular salivary gland cells and liver progenitor cells. Springerplus 3:183 (2014). ICC/IF ; Mouse . Read more (PubMed: 24790827) »
    See all 3 Publications for this product

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