Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2373(2)] to Myosin Phosphatase (phospho S903)
- Suitable for: WB
- Reacts with: Human
Product nameAnti-Myosin Phosphatase (phospho S903) antibody [EPR2373(2)]
See all Myosin Phosphatase primary antibodies
DescriptionRabbit monoclonal [EPR2373(2)] to Myosin Phosphatase (phospho S903)
Specificityab181181 detects Myosin Phosphatase phosphorylated at Serine 903 only.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Myosin Phosphatase aa 850-950 (phospho S903). The exact sequence is proprietary.
Database link: O14974
- Okadaic acid and Calyculin A treated HEK293 cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 115 kDa).|
FunctionRegulates myosin phosphatase activity.
Tissue specificityExpressed in striated muscles, specifically in type 2a fibers (at protein level).
Sequence similaritiesContains 6 ANK repeats.
Developmental stageInduced by 2-fold during pregnancy, including in abdominus rectus muscle.
DomainHeterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.
modificationsPhosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.
Cellular localizationCytoplasm. Along actomyosin filaments and stress fibers.
- Information by UniProt
- M130 antibody
- MBS antibody
- Myosin binding subunit antibody
All lanes : Anti-Myosin Phosphatase (phospho S903) antibody [EPR2373(2)] (ab181181) at 1/50000 dilution
Lane 1 : Okadaic acid and Calyculin A treated HEK293 cell lysate
Lane 2 : Untreated HEK293 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 115 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
ab181181 has not yet been referenced specifically in any publications.