Anti-Myosin Phosphatase (phospho T853) antibody (ab59203)
Key features and details
- Rabbit polyclonal to Myosin Phosphatase (phospho T853)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Myosin Phosphatase (phospho T853) antibody
See all Myosin Phosphatase primary antibodies -
Description
Rabbit polyclonal to Myosin Phosphatase (phospho T853) -
Host species
Rabbit -
Specificity
Detects human Myosin Phosphatase only when phosphorylated at threonine 853 (threonine 852 for mouse and threonine 855 for rat) -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human Myosin Phosphatase (phospho T853). (RSTPGV)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab59203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 115 kDa).
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 115 kDa). |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Regulates myosin phosphatase activity. -
Tissue specificity
Expressed in striated muscles, specifically in type 2a fibers (at protein level). -
Sequence similarities
Contains 6 ANK repeats. -
Developmental stage
Induced by 2-fold during pregnancy, including in abdominus rectus muscle. -
Domain
Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase. -
Post-translational
modificationsPhosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1. -
Cellular localization
Cytoplasm. Along actomyosin filaments and stress fibers. - Information by UniProt
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Database links
- Entrez Gene: 4659 Human
- Entrez Gene: 17931 Mouse
- Entrez Gene: 116670 Rat
- Omim: 602021 Human
- SwissProt: O14974 Human
- SwissProt: Q9DBR7 Mouse
- SwissProt: Q10728 Rat
- Unigene: 49582 Human
see all -
Alternative names
- M130 antibody
- MBS antibody
- Myosin binding subunit antibody
see all
Images
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All lanes : Anti-Myosin Phosphatase (phospho T853) antibody (ab59203) at 1/500 dilution
Lane 1 : NIH/3T3 cell extract
Lane 2 : NIH/3T3 cell extract with immunizing phosphopeptide
Predicted band size: 115 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin embedded human colon carcinoma tissue using ab59203 at 1/50 dilution. Samples were treated -/+ phosphopeptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab59203 has been referenced in 6 publications.
- Ma L et al. Leukocyte Rho kinase activity and serum cystatin C affect cardiovascular events in acute coronary syndrome. Medicine (Baltimore) 99:e20060 (2020). PubMed: 32664054
- Zhang M et al. Protective effects of 18ß-glycyrrhetinic acid on pulmonary arterial hypertension via regulation of Rho A/Rho kinsase pathway. Chem Biol Interact 311:108749 (2019). PubMed: 31325423
- Kao YH et al. Cyclic Mechanical Stretch Up-regulates Hepatoma-Derived Growth Factor Expression in Cultured Rat Aortic Smooth Muscle Cells. Biosci Rep N/A:N/A (2018). WB ; Rat . PubMed: 29467272
- Wang XQ et al. Resveratrol enhances vascular reactivity in mice following lipopolysaccharide challenge via the RhoA-ROCK-MLCP pathway. Exp Ther Med 14:308-316 (2017). PubMed: 28672931
- Cheng CI et al. The prognostic values of leukocyte rho kinase activity in acute ischemic stroke. Biomed Res Int 2014:214587 (2014). WB ; Human . PubMed: 24716192
- Montalvo J et al. ROCK1 & 2 perform overlapping and unique roles in angiogenesis and angiosarcoma tumor progression. Curr Mol Med 13:205-19 (2013). WB . PubMed: 22934846