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Signal Transduction Cytoskeleton / ECM Cytoskeleton Motor Proteins Myosin
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Anti-Myosin VIIa/MYO7A antibody (ab3481)

  • Datasheet
  • SDS
Reviews (9)Q&A (3)References (19)

Product price, shipping and contact information

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Western blot - Anti-Myosin VIIa/MYO7A antibody (ab3481)
  • Western blot - Anti-Myosin VIIa/MYO7A antibody (ab3481)
  • Flow Cytometry - Anti-Myosin VIIa/MYO7A antibody (ab3481)

Key features and details

  • Rabbit polyclonal to Myosin VIIa/MYO7A
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human Myosin VIIa/MYO7A protein (ab152555)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Myosin VIIa/MYO7A antibody
    See all Myosin VIIa/MYO7A primary antibodies
  • Description

    Rabbit polyclonal to Myosin VIIa/MYO7A
  • Host species

    Rabbit
  • Specificity

    Detects Myosin VIIa/MYO7A from mouse tissues as well as recombinant. By Western blot, this antibody detects an ~220 kDa protein representing myosin VIIa/MYO7A from mouse testes preparations. This antibody detects recombinant mouse myosin VIIa/MYO7A  overexpressed in Sf9 insect cell lysate.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Rat
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Mouse Myosin VIIa/MYO7A aa 16-31.
    Sequence:

    SGQEFDVPIGAVVKLC


    (Peptide available as ab4996)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

     This product was previously labelled as Myosin VIIa

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    Myosin VIIa is a member of the myosin superfamily of actin-based motor proteins. Defects in the myosin VIIa gene are responsible for hearing impairment in shaker-1 (sh1) mice and causes Usher syndrome IB in humans. Usher syndrome associates congenital deafness, vestibular dysfunction, and retinitis pigmentosa and is the most common form of combined deafness and blindness. Structural features of myosin VIIa protein include an ATP binding N-terminal motor domain, a central region which possess five light-chain binding (IQ) motifs, and a C-terminal domain with three myosin tail homology (MyTH4) and talin-like homology regions.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Myosin

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Myosin VIIa/MYO7A protein (ab152555)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Rat
WB
Mouse
Application Abreviews Notes
Flow Cyt
Use 3-5µg for 106 cells.
WB (1)
Use a concentration of 5 µg/ml. Detects a band of approximately 220 kDa.
Notes
Flow Cyt
Use 3-5µg for 106 cells.
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 220 kDa.

Target

  • Relevance

    Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. In retina, myosin VIIa may play a role in trafficking of ribbon-synaptic vesicle complexes and renewal of the outer photoreceptors disks. In inner ear, it may maintain the rigidity of stereocilia during the dynamic movements of the bundle.
  • Cellular localization

    Cytoplasmic, cytoskeleton
  • Database links

    • Entrez Gene: 17921 Mouse
    • Entrez Gene: 266714 Rat
    • SwissProt: P97479 Mouse
    • Unigene: 1403 Mouse
    • Unigene: 86641 Rat
    • Alternative names

      • Deafness autosomal dominant 11 antibody
      • Deafness autosomal recessive 2 antibody
      • DFNA11 antibody
      • DFNB 2 antibody
      • DFNB2 antibody
      • Myo7a antibody
      • Myosin 7a antibody
      • Myosin VIIA (Usher syndrome 1B (autosomal recessive, severe)) antibody
      • Myosin VIIa antibody
      • Myosin, unconventional, family VII, member A antibody
      • MYOVIIA antibody
      • MYU7A antibody
      • NSRD 2 antibody
      • NSRD2 antibody
      • Unconventional myosin VIIa antibody
      • Ush 1B antibody
      • Ush1b antibody
      • Usher syndrome 1B antibody
      see all

    Images

    • Western blot - Anti-Myosin VIIa/MYO7A antibody (ab3481)
      Western blot - Anti-Myosin VIIa/MYO7A antibody (ab3481)
      Anti-Myosin VIIa/MYO7A antibody (ab3481) at 5 µg/ml + Membrane enriched extracts of L6 at 30 µg with Skimmed milk at 5 %

      Secondary
      HRP conjugated goat anti-rabbit IgG (H+L) at 1/4000 dilution
    • Western blot - Anti-Myosin VIIa/MYO7A antibody (ab3481)
      Western blot - Anti-Myosin VIIa/MYO7A antibody (ab3481)

      Western blot detection of Myosin VIIa/MYO7A in mouse testes tissue extract using ab3481.

    • Flow Cytometry - Anti-Myosin VIIa/MYO7A antibody (ab3481)
      Flow Cytometry - Anti-Myosin VIIa/MYO7A antibody (ab3481)

      ab3481 staining Myosin VIIa/MYO7A (red histogram) on L6 cells by Flow Cytometry. Cells were fixed with 70% ethonal, permeabilized with Triton and blocked with 5% BSA for 30 minutes at room temperature. The sample was incubated with the primary antibody (3-5 ug/10^6 in 2.5% BSA) for 2 hours at room temperature. An Alexa fluor® 488-conjugated Goat anti-rabbit IgG (1/400) was used as the secondary antibody. Purple histogram represents unstained control, pink histogram represents rabbit isotype control and green histogram represents no primary antibody control

       

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (19)

    Publishing research using ab3481? Please let us know so that we can cite the reference in this datasheet.

    ab3481 has been referenced in 19 publications.

    • Mei H  et al. Increased mitochondrial DNA copy number protects hair cells and HEI-OC1 cells against drug-induced apoptosis. Mol Med Rep 21:338-346 (2020). PubMed: 31939628
    • Lee SY  et al. Outcomes of Peptide Vaccine GV1001 Treatment in a Murine Model of Acute Noise-Induced Hearing Loss. Antioxidants (Basel) 9:N/A (2020). PubMed: 32012778
    • Li S  et al. FGF22 promotes generation of ribbon synapses through downregulating MEF2D. Aging (Albany NY) 12:6456-6466 (2020). PubMed: 32271716
    • Wen Q  et al. Myosin VIIa Supports Spermatid/Organelle Transport and Cell Adhesion During Spermatogenesis in the Rat Testis. Endocrinology 160:484-503 (2019). PubMed: 30649248
    • Xu W  et al. Endothelial FAM3A positively regulates post-ischaemic angiogenesis. EBioMedicine 43:32-42 (2019). PubMed: 31000420
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 12 Abreviews or Q&A

    IHC - Wholemount abreview for Anti-Myosin VIIa antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Cochlea)
    Specification
    Cochlea
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 10 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myosin VIIa antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Whole Mouse Foetus Section)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer
    Permeabilization
    Yes - Triton X-100
    Specification
    Whole Mouse Foetus Section
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 16 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin VIIa antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Dog Cell (MDCK II)
    Specification
    MDCK II
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% TX100
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Herr Dr. Vladimir Milenkovic

    Verified customer

    Submitted Nov 27 2009

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin VIIa antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Retinal Pigment Epithelium primary cells)
    Specification
    Retinal Pigment Epithelium primary cells
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.5% TX100
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Herr Dr. Vladimir Milenkovic

    Verified customer

    Submitted Nov 26 2009

    Question

    Expiry data and availability

    Read More

    Abcam community

    Verified customer

    Asked on Nov 18 2009

    Answer

    Thank you for your enquiry. There is no stated expiration date for this antibody. There is one year expiration date from the date of purchase, however the Abpromise will be still effective for only 120 days after purchase. This antibody is unlikely to be be discontinued as the sales for the last two years have been consistent. I hope this information helps. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Nov 18 2009

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin VIIa antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Primary cell culture, retinal pigment epithelium)
    Specification
    Primary cell culture, retinal pigment epithelium
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% TX100
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Herr Dr. Vladimir Milenkovic

    Verified customer

    Submitted Dec 02 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin VIIa antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Hamster Cell (CHO, chinese hamster ovary cells)
    Specification
    CHO, chinese hamster ovary cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton X100
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Herr Dr. Vladimir Milenkovic

    Verified customer

    Submitted Dec 02 2008

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myosin VIIa antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (ear cross sections)
    Specification
    ear cross sections
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Enzymatic
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5%
    Read More

    Mrs. Chantal Longo-Guess

    Verified customer

    Submitted Jun 10 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin VIIa antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Pig Cell (Primary cell culture, retinal pigment epithelium)
    Specification
    Primary cell culture, retinal pigment epithelium
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% TX100
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Herr Dr. Vladimir Milenkovic

    Verified customer

    Submitted Jun 05 2008

    Western blot abreview for Anti-Myosin VIIa antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Pig Cell lysate - whole cell (Pig RPE cells)
    Loading amount
    25 µg
    Specification
    Pig RPE cells
    Gel Running Conditions
    Reduced Denaturing (7%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Herr Dr. Vladimir Milenkovic

    Verified customer

    Submitted Jun 03 2008

    1-10 of 12 Abreviews or Q&A

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