Anti-Myosin VIIa/MYO7A antibody (ab3481)
- Datasheet
- References (12)
- Protocols
Overview
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Product nameAnti-Myosin VIIa/MYO7A antibody
See all Myosin VIIa/MYO7A primary antibodies -
DescriptionRabbit polyclonal to Myosin VIIa/MYO7A
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Host speciesRabbit
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Specificity
Detects Myosin VIIa/MYO7A from mouse tissues as well as recombinant. By Western blot, this antibody detects an ~220 kDa protein representing myosin VIIa/MYO7A from mouse testes preparations. This antibody detects recombinant mouse myosin VIIa/MYO7A overexpressed in Sf9 insect cell lysate.
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Tested applicationsSuitable for: IHC-P, Flow Cyt, ICC, IP, WB, ICC/IF, IHC-Frmore details
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Species reactivityReacts with: Mouse, Rat, Guinea pig, Hamster, Cow, Dog, Human, Pig
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Immunogen
Synthetic peptide corresponding to Mouse Myosin VIIa/MYO7A aa 16-31.
Sequence:SGQEFDVPIGAVVKLC
(Peptide available asab4996) -
Positive control
- WB: mouse testes lysate IHC-P: mouse ear
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General notes
This product was previously labelled as Myosin VIIa
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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Primary antibody notesMyosin VIIa is a member of the myosin superfamily of actin-based motor proteins. Defects in the myosin VIIa gene are responsible for hearing impairment in shaker-1 (sh1) mice and causes Usher syndrome IB in humans. Usher syndrome associates congenital deafness, vestibular dysfunction, and retinitis pigmentosa and is the most common form of combined deafness and blindness. Structural features of myosin VIIa protein include an ATP binding N-terminal motor domain, a central region which possess five light-chain binding (IQ) motifs, and a C-terminal domain with three myosin tail homology (MyTH4) and talin-like homology regions.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab3481 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| IHC-P | 1/500. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. | |
| Flow Cyt | Use 3-5µg for 106 cells. | |
| ICC | 1/50. | |
| IP | Use at an assay dependent concentration. | |
| WB | Use a concentration of 5 µg/ml. Detects a band of approximately 220 kDa.Can be blocked with Myosin VIIa/MYO7A peptide (ab4996). | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 19429027 | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 20461409 |
Target
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RelevanceMyosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. In retina, myosin VIIa may play a role in trafficking of ribbon-synaptic vesicle complexes and renewal of the outer photoreceptors disks. In inner ear, it may maintain the rigidity of stereocilia during the dynamic movements of the bundle.
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Cellular localizationCytoplasmic, cytoskeleton
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Database links
- Entrez Gene: 4647 Human
- Entrez Gene: 17921 Mouse
- Entrez Gene: 397373 Pig
- Entrez Gene: 266714 Rat
- Omim: 276903 Human
- SwissProt: Q13402 Human
- SwissProt: P97479 Mouse
- Unigene: 370421 Human
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Alternative names
- Deafness autosomal dominant 11 antibody
- Deafness autosomal recessive 2 antibody
- DFNA11 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Myosin VIIa/MYO7A antibody (ab3481)This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovicab3481 staining primary cell culture of Pig retinal pigment epithelium by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 5% serum for 20 minutes at 25°C. The primary antibody was diluted 1/500 and incubated with the sample for 16 hours at 4°C. An Alexa Fluor® 148 conjugated goat anti-rabbit antibody, diluted 1/750, was used as the secondary.
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Western blot - Anti-Myosin VIIa/MYO7A antibody (ab3481)Anti-Myosin VIIa/MYO7A antibody (ab3481) at 5 µg/ml + Membrane enriched extracts of L6 at 30 µg with Skimmed milk at 5 %
Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/4000 dilution -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin VIIa/MYO7A antibody (ab3481)Image from Miller KA et al., PLoS One. 2012;7(12):e51284. Fig 8.; doi: 10.1371/journal.pone.0051284. Epub 2012 Dec 12(G) Histochemical staining of mouse P5 cochlear sensory epithelia inner hair cells (IHC) and outer hair cells (OHC) with Alexa Fluor 488® phalloidin. (H) Immunohistochemical staining of mouse P5 cochlear sensory epithelia with rabbit IgG as an isotype control and an Alexa Fluor 594®-conjugated goat anti-rabbit antibody as the secondary antibody (dilution 1/2500). The scale bars represent 8 μm.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin VIIa/MYO7A antibody (ab3481)Image from Miller KA et al., PLoS One. 2012;7(12):e51284. Fig 8.; doi: 10.1371/journal.pone.0051284. Epub 2012 Dec 12(A) Histochemical staining of mouse P5 cochlear sensory epithelia inner hair cells (IHC) and outer hair cells (OHC) with Alexa Fluor 488® phalloidin. (B) Immunohistochemical staining of mouse P5 cochlear sensory epithelia with ab3481 as the primary antibody (dilution 1/900) and an Alexa Fluor 594®-conjugated goat anti-rabbit antibody as the secondary antibody (dilution 1/2500). The scale bars represent 8 μm.
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Immunocytochemistry/ Immunofluorescence - Anti-Myosin VIIa/MYO7A antibody (ab3481)This image was kindly supplied by Dr Vladimir Milenkovic by Abreviewab3481 at a 1/500 dilution staining Myosin VIIa/MYO7A in Mouse retinal pigment epithelium primary cells by Immunocytochemistry/ Immunofluorescence, incubated for 16 hours at 4°C in 1% goat serum, 0.1% Triton X-100, 1X PBS. Fixed in formalin. Permeabilized using 0.5% Triton X-100. Blocked with 5% serum for 20 minutes at 25°C. Secondary used at 1/500 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 488.
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Western blot - Anti-Myosin VIIa/MYO7A antibody (ab3481)Western blot detection of Myosin VIIa/MYO7A in mouse testes tissue extract using ab3481.
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Flow Cytometry - Anti-Myosin VIIa/MYO7A antibody (ab3481)ab3481 staining Myosin VIIa/MYO7A (red histogram) on L6 cells by Flow Cytometry. Cells were fixed with 70% ethonal, permeabilized with Triton and blocked with 5% BSA for 30 minutes at room temperature. The sample was incubated with the primary antibody (3-5 ug/10^6 in 2.5% BSA) for 2 hours at room temperature. An Alexa fluor® 488-conjugated Goat anti-rabbit IgG (1/400) was used as the secondary antibody. Purple histogram represents unstained control, pink histogram represents rabbit isotype control and green histogram represents no primary antibody control
Protocols
References
This product has been referenced in:
- Fan F et al. Exophilin-8 assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa. Elife 6:N/A (2017). Read more (PubMed: 28673385) »
- Santos AC et al. Cochlear epithelial of dog fetuses: a new source of multipotent stem cells. Cytotechnology 69:179-189 (2017). Read more (PubMed: 28074389) »
