Anti-Myosin VIIa/MYO7A antibody (ab3481)
Key features and details
- Rabbit polyclonal to Myosin VIIa/MYO7A
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Myosin VIIa/MYO7A antibody
See all Myosin VIIa/MYO7A primary antibodies -
Description
Rabbit polyclonal to Myosin VIIa/MYO7A -
Host species
Rabbit -
Specificity
Detects Myosin VIIa/MYO7A from mouse tissues as well as recombinant. By Western blot, this antibody detects an ~220 kDa protein representing myosin VIIa/MYO7A from mouse testes preparations. This antibody detects recombinant mouse myosin VIIa/MYO7A overexpressed in Sf9 insect cell lysate.
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Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt RatWB Mouse
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Immunogen
Synthetic peptide corresponding to Mouse Myosin VIIa/MYO7A aa 16-31.
Sequence:SGQEFDVPIGAVVKLC
(Peptide available asab4996) -
General notes
This product was previously labelled as Myosin VIIa
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
Myosin VIIa is a member of the myosin superfamily of actin-based motor proteins. Defects in the myosin VIIa gene are responsible for hearing impairment in shaker-1 (sh1) mice and causes Usher syndrome IB in humans. Usher syndrome associates congenital deafness, vestibular dysfunction, and retinitis pigmentosa and is the most common form of combined deafness and blindness. Structural features of myosin VIIa protein include an ATP binding N-terminal motor domain, a central region which possess five light-chain binding (IQ) motifs, and a C-terminal domain with three myosin tail homology (MyTH4) and talin-like homology regions. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3481 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Rat
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| WB |
Mouse
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| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 3-5µg for 106 cells.
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| WB | (1) |
Use a concentration of 5 µg/ml. Detects a band of approximately 220 kDa.
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| Notes |
|---|
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Flow Cyt
Use 3-5µg for 106 cells. |
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WB
Use a concentration of 5 µg/ml. Detects a band of approximately 220 kDa. |
Target
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Relevance
Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. In retina, myosin VIIa may play a role in trafficking of ribbon-synaptic vesicle complexes and renewal of the outer photoreceptors disks. In inner ear, it may maintain the rigidity of stereocilia during the dynamic movements of the bundle. -
Cellular localization
Cytoplasmic, cytoskeleton -
Database links
- Entrez Gene: 17921 Mouse
- Entrez Gene: 266714 Rat
- SwissProt: P97479 Mouse
- Unigene: 1403 Mouse
- Unigene: 86641 Rat
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Alternative names
- Deafness autosomal dominant 11 antibody
- Deafness autosomal recessive 2 antibody
- DFNA11 antibody
see all
Images
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Western blot - Anti-Myosin VIIa/MYO7A antibody (ab3481)Anti-Myosin VIIa/MYO7A antibody (ab3481) at 5 µg/ml + Membrane enriched extracts of L6 at 30 µg with Skimmed milk at 5 %
Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/4000 dilution -
Western blot - Anti-Myosin VIIa/MYO7A antibody (ab3481)Western blot detection of Myosin VIIa/MYO7A in mouse testes tissue extract using ab3481.
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Flow Cytometry - Anti-Myosin VIIa/MYO7A antibody (ab3481)ab3481 staining Myosin VIIa/MYO7A (red histogram) on L6 cells by Flow Cytometry. Cells were fixed with 70% ethonal, permeabilized with Triton and blocked with 5% BSA for 30 minutes at room temperature. The sample was incubated with the primary antibody (3-5 ug/10^6 in 2.5% BSA) for 2 hours at room temperature. An Alexa fluor® 488-conjugated Goat anti-rabbit IgG (1/400) was used as the secondary antibody. Purple histogram represents unstained control, pink histogram represents rabbit isotype control and green histogram represents no primary antibody control
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab3481 has been referenced in 19 publications.
- Mei H et al. Increased mitochondrial DNA copy number protects hair cells and HEI-OC1 cells against drug-induced apoptosis. Mol Med Rep 21:338-346 (2020). PubMed: 31939628
- Lee SY et al. Outcomes of Peptide Vaccine GV1001 Treatment in a Murine Model of Acute Noise-Induced Hearing Loss. Antioxidants (Basel) 9:N/A (2020). PubMed: 32012778
- Li S et al. FGF22 promotes generation of ribbon synapses through downregulating MEF2D. Aging (Albany NY) 12:6456-6466 (2020). PubMed: 32271716
- Wen Q et al. Myosin VIIa Supports Spermatid/Organelle Transport and Cell Adhesion During Spermatogenesis in the Rat Testis. Endocrinology 160:484-503 (2019). PubMed: 30649248
- Xu W et al. Endothelial FAM3A positively regulates post-ischaemic angiogenesis. EBioMedicine 43:32-42 (2019). PubMed: 31000420