Overview

  • Product name
    Anti-Myosin VIIa/MYO7A antibody
    See all Myosin VIIa/MYO7A primary antibodies
  • Description
    Rabbit polyclonal to Myosin VIIa/MYO7A
  • Host species
    Rabbit
  • Specificity

    Detects Myosin VIIa/MYO7A from mouse tissues as well as recombinant. By Western blot, this antibody detects an ~220 kDa protein representing myosin VIIa/MYO7A from mouse testes preparations. This antibody detects recombinant mouse myosin VIIa/MYO7A  overexpressed in Sf9 insect cell lysate.

  • Tested applications
    Suitable for: IHC-P, Flow Cyt, ICC, IP, WB, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Hamster, Cow, Dog, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to Mouse Myosin VIIa/MYO7A aa 16-31.
    Sequence:

    SGQEFDVPIGAVVKLC


    (Peptide available as ab4996)

  • Positive control
    • WB: mouse testes lysate IHC-P: mouse ear
  • General notes

     This product was previously labelled as Myosin VIIa

     

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Myosin VIIa is a member of the myosin superfamily of actin-based motor proteins. Defects in the myosin VIIa gene are responsible for hearing impairment in shaker-1 (sh1) mice and causes Usher syndrome IB in humans. Usher syndrome associates congenital deafness, vestibular dysfunction, and retinitis pigmentosa and is the most common form of combined deafness and blindness. Structural features of myosin VIIa protein include an ATP binding N-terminal motor domain, a central region which possess five light-chain binding (IQ) motifs, and a C-terminal domain with three myosin tail homology (MyTH4) and talin-like homology regions.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
Flow Cyt Use 3-5µg for 106 cells.
ICC 1/50.
IP Use at an assay dependent concentration.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 220 kDa.Can be blocked with Myosin VIIa/MYO7A peptide (ab4996).
ICC/IF Use at an assay dependent concentration. PubMed: 19429027
IHC-Fr Use at an assay dependent concentration. PubMed: 20461409

Target

  • Relevance
    Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. In retina, myosin VIIa may play a role in trafficking of ribbon-synaptic vesicle complexes and renewal of the outer photoreceptors disks. In inner ear, it may maintain the rigidity of stereocilia during the dynamic movements of the bundle.
  • Cellular localization
    Cytoplasmic, cytoskeleton
  • Database links
  • Alternative names
    • Deafness autosomal dominant 11 antibody
    • Deafness autosomal recessive 2 antibody
    • DFNA11 antibody
    • DFNB 2 antibody
    • DFNB2 antibody
    • Myo7a antibody
    • Myosin 7a antibody
    • Myosin VIIA (Usher syndrome 1B (autosomal recessive, severe)) antibody
    • Myosin VIIa antibody
    • Myosin, unconventional, family VII, member A antibody
    • MYOVIIA antibody
    • MYU7A antibody
    • NSRD 2 antibody
    • NSRD2 antibody
    • Unconventional myosin VIIa antibody
    • Ush 1B antibody
    • Ush1b antibody
    • Usher syndrome 1B antibody
    see all

Images

  • ab3481 staining primary cell culture of Pig retinal pigment epithelium by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 5% serum for 20 minutes at 25°C.  The primary antibody was diluted 1/500 and incubated with the sample for 16 hours at 4°C.  An Alexa Fluor® 148 conjugated goat anti-rabbit antibody, diluted 1/750, was used as the secondary.

    See Abreview

  • Anti-Myosin VIIa/MYO7A antibody (ab3481) at 5 µg/ml + Membrane enriched extracts of L6 at 30 µg with Skimmed milk at 5 %

    Secondary
    HRP conjugated goat anti-rabbit IgG (H+L) at 1/4000 dilution
  • (G) Histochemical staining of mouse P5 cochlear sensory epithelia inner hair cells (IHC) and outer hair cells (OHC) with Alexa Fluor 488® phalloidin. (H) Immunohistochemical staining of mouse P5 cochlear sensory epithelia with rabbit IgG as an isotype control and an Alexa Fluor 594®-conjugated goat anti-rabbit antibody as the secondary antibody (dilution 1/2500). The scale bars represent 8 μm.

  • (A) Histochemical staining of mouse P5 cochlear sensory epithelia inner hair cells (IHC) and outer hair cells (OHC) with Alexa Fluor 488® phalloidin.  (B) Immunohistochemical staining of mouse P5 cochlear sensory epithelia with ab3481 as the primary antibody (dilution 1/900) and an Alexa Fluor 594®-conjugated goat anti-rabbit antibody as the secondary antibody (dilution 1/2500). The scale bars represent 8 μm.

  • ab3481 at a 1/500 dilution staining Myosin VIIa/MYO7A in Mouse retinal pigment epithelium primary cells by Immunocytochemistry/ Immunofluorescence, incubated for 16 hours at 4°C in 1% goat serum, 0.1% Triton X-100, 1X PBS. Fixed in formalin. Permeabilized using 0.5% Triton X-100. Blocked with 5% serum for 20 minutes at 25°C. Secondary used at 1/500 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 488.

    See Abreview

  • Western blot detection of Myosin VIIa/MYO7A in mouse testes tissue extract using ab3481.

  • ab3481 staining Myosin VIIa/MYO7A (red histogram) on L6 cells by Flow Cytometry. Cells were fixed with 70% ethonal, permeabilized with Triton and blocked with 5% BSA for 30 minutes at room temperature. The sample was incubated with the primary antibody (3-5 ug/10^6 in 2.5% BSA) for 2 hours at room temperature. An Alexa fluor® 488-conjugated Goat anti-rabbit IgG (1/400) was used as the secondary antibody. Purple histogram represents unstained control, pink histogram represents rabbit isotype control and green histogram represents no primary antibody control

     

References

This product has been referenced in:
  • Fan F  et al. Exophilin-8 assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa. Elife 6:N/A (2017). Read more (PubMed: 28673385) »
  • Santos AC  et al. Cochlear epithelial of dog fetuses: a new source of multipotent stem cells. Cytotechnology 69:179-189 (2017). Read more (PubMed: 28074389) »
See all 12 Publications for this product

Customer reviews and Q&As

1-3 of 3 Q&A

Question
Answer

Thank you for your enquiry. There is no stated expiration date for this antibody. There is one year expiration date from the date of purchase, however the Abpromise will be still effective for only 120 days after purchase. This antibody is unlikely to be be discontinued as the sales for the last two years have been consistent. I hope this information helps. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry. All the information that we have regarding this antibody is located on the online datasheet and we are unaware if the sequence is also conserved in zebrafish.

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Answer

Thank you for your enquiry. This antibody has not yet been tested for use with immunofluorescence. Should you decide to go ahead and test this antibody, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift voucher. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement.

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