Western blot abreview for Anti-Myosin VIIa antibody

Excellent
Abreviews
Application
Western blot
Sample
Pig Cell lysate - whole cell (Pig RPE cells)
Loading amount
25 µg
Specification
Pig RPE cells
Gel Running Conditions
Reduced Denaturing (7%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1X PBS/Tween, 5% milk

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL+
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: ca 250 kDa Non-specific: ca 150 kDa

Additional data

Additional Notes
Line 1: HEK293 cells lysate
Line 2: pig RPE cells lysate

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Jun 03 2008

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