Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin VIIa antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary cell culture, retinal pigment epithelium)
Specification
Primary cell culture, retinal pigment epithelium
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TX100
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% goat serum, 0.1%TX100, 1XPBS

Secondary antibody

Name
Non-Abcam antibody was used: anti rabbit Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/750

Additional data

Additional Notes
Primary cell culture from rat RPE cells:
Nuclei were counterstained with DAPI (blue), and myosin7a with pAb ab3481 (green).

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Dec 02 2008

Sign up