Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin VIIa antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (CHO, chinese hamster ovary cells)
Specification
CHO, chinese hamster ovary cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X100
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% goat serum, 0.1%TX100, 1XPBS

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti rabbit Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/750

Additional data

Additional Notes
CHO, chinese hamster ovary cells.
Nuclei were counterstained with DAPI (blue), and myosin7a with pAb ab3481 (green).

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Dec 02 2008

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