Recombinant Anti-Myosin VIIa/MYO7A antibody [EPR7497] (ab150386)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7497] to Myosin VIIa/MYO7A
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Myosin VIIa/MYO7A antibody [EPR7497]
See all Myosin VIIa/MYO7A primary antibodies -
Description
Rabbit monoclonal [EPR7497] to Myosin VIIa/MYO7A -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt (Intra)more details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Myosin VIIa/MYO7A aa 1-100. The exact sequence is proprietary.
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Positive control
- Rat and mouse testis lysates; Y79 and A97 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7497 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 254 kDa.
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ICC/IF |
1/100 - 1/250.
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Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 254 kDa. |
ICC/IF
1/100 - 1/250. |
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. In retina, myosin VIIa may play a role in trafficking of ribbon-synaptic vesicle complexes and renewal of the outer photoreceptors disks. In inner ear, it may maintain the rigidity of stereocilia during the dynamic movements of the bundle. -
Cellular localization
Cytoplasmic, cytoskeleton -
Database links
- Entrez Gene: 4647 Human
- Entrez Gene: 17921 Mouse
- Entrez Gene: 266714 Rat
- Omim: 276903 Human
- SwissProt: Q13402 Human
- SwissProt: P97479 Mouse
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Alternative names
- Deafness autosomal dominant 11 antibody
- Deafness autosomal recessive 2 antibody
- DFNA11 antibody
see all
Images
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All lanes : Anti-Myosin VIIa/MYO7A antibody [EPR7497] (ab150386) at 1/1000 dilution
Lane 1 : Y79 (Human retinoblastoma cell line) cell lysate
Lane 2 : Rat testis tissue lysate
Lane 3 : Mouse testis tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat-anti rabbit antibody at 1/2000 dilution
Predicted band size: 254 kDa -
Immunofluorescence analysis of A97 cells labeling Myosin VIIa/MYO7A with ab150386 at a 1/100 dilution.
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Intracellular flow cytometric analysis of permeabilized Y79 cells labeling Myosin VIIa/MYO7A with ab150386 at a 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab150386 has been referenced in 5 publications.
- Al-Choboq J et al. Usher Syndrome Belongs to the Genetic Diseases Associated with Radiosensitivity: Influence of the ATM Protein Kinase. Int J Mol Sci 23:N/A (2022). PubMed: 35163494
- Zhang YL et al. Small activating RNA activation of ATOH1 promotes regeneration of human inner ear hair cells. Bioengineered 13:6729-6739 (2022). PubMed: 35246011
- Wu Y et al. Pitavastatin protects against neomycin-induced ototoxicity through inhibition of endoplasmic reticulum stress. Front Mol Neurosci 15:963083 (2022). PubMed: 35992197
- Peng T et al. miR-125/CDK2 axis in cochlear progenitor cell proliferation. Mol Med Rep 23:N/A (2021). PubMed: 33300064
- Kucharava K et al. Sodium-hydrogen exchanger 6 (NHE6) deficiency leads to hearing loss, via reduced endosomal signalling through the BDNF/Trk pathway. Sci Rep 10:3609 (2020). PubMed: 32107410