Overview

  • Product name

    Anti-Myosin VIIa/MYO7A antibody [EPR7497]
    See all Myosin VIIa/MYO7A primary antibodies
  • Description

    Rabbit monoclonal [EPR7497] to Myosin VIIa/MYO7A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Myosin VIIa/MYO7A aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Rat and mouse testis lysates; Y79 and A97 cells
  • General notes

     

     

     This product was previously labelled as Myosin VIIa

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7497
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 254 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Relevance

      Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. In retina, myosin VIIa may play a role in trafficking of ribbon-synaptic vesicle complexes and renewal of the outer photoreceptors disks. In inner ear, it may maintain the rigidity of stereocilia during the dynamic movements of the bundle.
    • Cellular localization

      Cytoplasmic, cytoskeleton
    • Database links

    • Alternative names

      • Deafness autosomal dominant 11 antibody
      • Deafness autosomal recessive 2 antibody
      • DFNA11 antibody
      • DFNB 2 antibody
      • DFNB2 antibody
      • Myo7a antibody
      • Myosin 7a antibody
      • Myosin VIIA (Usher syndrome 1B (autosomal recessive, severe)) antibody
      • Myosin VIIa antibody
      • Myosin, unconventional, family VII, member A antibody
      • MYOVIIA antibody
      • MYU7A antibody
      • NSRD 2 antibody
      • NSRD2 antibody
      • Unconventional myosin VIIa antibody
      • Ush 1B antibody
      • Ush1b antibody
      • Usher syndrome 1B antibody
      see all

    Images

    • All lanes : Anti-Myosin VIIa/MYO7A antibody [EPR7497] (ab150386) at 1/1000 dilution

      Lane 1 : Y79 cell lysate
      Lane 2 : rat testis cell lysate
      Lane 3 : mouse testis cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat-anti rabbit antibody at 1/2000 dilution

      Predicted band size: 254 kDa

    • Immunofluorescence analysis of A97 cells labeling Myosin VIIa/MYO7A with ab150386 at a 1/100 dilution.

    • Flow cytometric analysis of permeabilized Y79 cells labeling Myosin VIIa/MYO7A with ab150386 at a 1/100 dilution.

    References

    ab150386 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab150386.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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