Product nameAnti-Myosin VIIa/MYO7A antibody [EPR7497]
See all Myosin VIIa/MYO7A primary antibodies
DescriptionRabbit monoclonal [EPR7497] to Myosin VIIa/MYO7A
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Myosin VIIa/MYO7A aa 1-100. The exact sequence is proprietary.
- Rat and mouse testis lysates; Y79 and A97 cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab150386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 254 kDa.|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceMyosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. In retina, myosin VIIa may play a role in trafficking of ribbon-synaptic vesicle complexes and renewal of the outer photoreceptors disks. In inner ear, it may maintain the rigidity of stereocilia during the dynamic movements of the bundle.
Cellular localizationCytoplasmic, cytoskeleton
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All lanes : Anti-Myosin VIIa/MYO7A antibody [EPR7497] (ab150386) at 1/1000 dilution
Lane 1 : Y79 cell lysate
Lane 2 : rat testis cell lysate
Lane 3 : mouse testis cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat-anti rabbit antibody at 1/2000 dilution
Predicted band size: 254 kDa
Immunofluorescence analysis of A97 cells labeling Myosin VIIa/MYO7A with ab150386 at a 1/100 dilution.
Flow cytometric analysis of permeabilized Y79 cells labeling Myosin VIIa/MYO7A with ab150386 at a 1/100 dilution.
ab150386 has not yet been referenced specifically in any publications.