Key features and details
- Rabbit polyclonal to MYOZ3
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MYOZ3 antibody
DescriptionRabbit polyclonal to MYOZ3
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Dog
- Human skeletal muscle tissue; Human tonsil lysate
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122540 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform heat mediated antigen retrieval at pH6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
FunctionMyozenins may serve as intracellular binding proteins involved in linking Z line proteins such as alpha-actinin, gamma-filamin, TCAP/telethonin, LDB3/ZASP and localizing calcineurin signaling to the sarcomere. Plays an important role in the modulation of calcineurin signaling. May play a role in myofibrillogenesis.
Tissue specificityExpressed specifically in skeletal muscle. Not detected in heart.
Sequence similaritiesBelongs to the myozenin family.
Cellular localizationCytoplasm > myofibril > sarcomere > Z line. Localized at the Z-line of skeletal muscle.
- Information by UniProt
- Calsarcin-3 antibody
- CS 3 antibody
- CS3 antibody
Immunofluorescent staining of Human cell line U-251 MG shows positivity in cytoplasm. Recommended concentration of ab122540 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122540, at 1/350 dilution, staining MYOZ3 in paraffin-embedded Human skeletal muscle tissue by Immunohistochemistry, showing distinct cytoplasmic positivity in myocytes.
All lanes : Anti-MYOZ3 antibody (ab122540) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
ab122540 has not yet been referenced specifically in any publications.