Anti-MYPN antibody (ab233039)
Key features and details
- Rabbit polyclonal to MYPN
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-MYPN antibody -
Description
Rabbit polyclonal to MYPN -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse -
Immunogen
Recombinant fragment (His-tag) corresponding to Human MYPN aa 900-1350. (Expressed in E.coli).
Database link: Q86TC9 -
Positive control
- WB: Pig heart and skeletal muscle lysates; Recombinant human MYPN protein. IHC-P: Human skeletal muscle tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab233039 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 145 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 145 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Component of the sarcomere that tethers together nebulin (skeletal muscle) and nebulette (cardiac muscle) to alpha-actinin, at the Z lines. -
Tissue specificity
Expressed in adult skeletal muscle and fetal heart. -
Sequence similarities
Belongs to the myotilin/palladin family.
Contains 5 Ig-like (immunoglobulin-like) domains. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasm > myofibril > sarcomere. Bound to sarcomere both at the Z-line periphery and in the central I-band region. - Information by UniProt
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Database links
- Entrez Gene: 84665 Human
- Entrez Gene: 68802 Mouse
- Entrez Gene: 100624901 Pig
- Omim: 608517 Human
- SwissProt: Q86TC9 Human
- SwissProt: Q5DTJ9 Mouse
- Unigene: 55205 Human
- Unigene: 259767 Mouse
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Alternative names
- 145 kDa sarcomeric protein antibody
- MYOP antibody
- Myopalladin antibody
see all
Images
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Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for MYPN using ab233039 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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All lanes : Anti-MYPN antibody (ab233039) at 2 µg/ml
Lane 1 : Pig heart lysate
Lane 2 : Pig skeletal muscle lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/1000 dilution
Predicted band size: 145 kDa -
Anti-MYPN antibody (ab233039) at 2 µg/ml + Recombinant human MYPN protein
Predicted band size: 145 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab233039 has not yet been referenced specifically in any publications.