Key features and details
- Rabbit polyclonal to MYPN
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-MYPN antibody
See all MYPN primary antibodies
DescriptionRabbit polyclonal to MYPN
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Mouse
Recombinant fragment (His-tag) corresponding to Human MYPN aa 945-1320. (Expressed in E.coli).
PIFDKRLKHFRVTEGSPVTFTCKIVGIPVPKVYWFKDGKQISKRNEHCKM RREGDGTCSLHIESTTSDDDGNYTIMAANPQGRISCSGHLMVQSLPIRSR LTSAGQSHRGRSRVQERDKEPLQERFFRPHFLQAPGDMVAHEGRLCRLDC KVSGLPPPELTWLLNGQPVLPDASHKMLVRETGVHSLLIDPLTQRDAGTY KCIATNKTGQNSFSLELSVVAKEVKKAPVILEKLQNCGVPEGHPVRLECR VIGMPPPVFYWKKDNETIPCTRERISMHQDTTGYACLLIQPAKKSDAGWY TLSAKNEAGIVSCTARLDIYAQWHHQIPPPMSVRPSGSRYGSLTSKGLDI FSAFSSMESTMVYSCSSRSVVESDEL
Database link: Q86TC9
- WB: Pig heart and skeletal muscle lysates; Recombinant human MYPN protein. IHC-P: Human skeletal muscle tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab233039 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 145 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionComponent of the sarcomere that tethers together nebulin (skeletal muscle) and nebulette (cardiac muscle) to alpha-actinin, at the Z lines.
Tissue specificityExpressed in adult skeletal muscle and fetal heart.
Sequence similaritiesBelongs to the myotilin/palladin family.
Contains 5 Ig-like (immunoglobulin-like) domains.
Cellular localizationCytoplasm. Nucleus. Cytoplasm > myofibril > sarcomere. Bound to sarcomere both at the Z-line periphery and in the central I-band region.
- Information by UniProt
- 145 kDa sarcomeric protein antibody
- MYOP antibody
- Myopalladin antibody
Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for MYPN using ab233039 at 20 µg/ml in immunohistochemical analysis. DAB staining.
All lanes : Anti-MYPN antibody (ab233039) at 2 µg/ml
Lane 1 : Pig heart lysate
Lane 2 : Pig skeletal muscle lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/1000 dilution
Predicted band size: 145 kDa
Anti-MYPN antibody (ab233039) at 2 µg/ml + Recombinant human MYPN protein
Predicted band size: 145 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233039 has not yet been referenced specifically in any publications.