Key features and details
- Goat polyclonal to MYRIP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MYRIP antibody
See all MYRIP primary antibodies
DescriptionGoat polyclonal to MYRIP
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
A molecular complex composed of Rab27A, MYRIP and myosin VIIa bridges retinal melanosomes to the actin cytoskeleton and thereby mediates the local trafficking of these organelles. The defect of this molecular complex is likely to account for the perinuclear mislocalization of the melanosomes observed in the retinal pigment epithelium cells of myosin VIIa-defective mice.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Primary antibody notesA molecular complex composed of Rab27A, MYRIP and myosin VIIa bridges retinal melanosomes to the actin cytoskeleton and thereby mediates the local trafficking of these organelles. The defect of this molecular complex is likely to account for the perinuclear mislocalization of the melanosomes observed in the retinal pigment epithelium cells of myosin VIIa-defective mice.
Our Abpromise guarantee covers the use of ab10149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 96 kDa.
1 hour primary incubation is recommended for this product.
FunctionRab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor proteins MYO5A and MYO7A. May link RAB27A-containing vesicles to actin filaments.
Tissue specificityDetected in brain, skin, heart, adrenal medulla, pancreas, intestine, liver, kidney, muscle and testis.
Sequence similaritiesContains 1 FYVE-type zinc finger.
Contains 1 RabBD (Rab-binding) domain.
Cellular localizationCytoplasm. In pre- and post-synaptic areas in photoreceptor cells and in the basal microvilli of retinal pigment epithelium cells. Associated with melanosomes. Colocalizes with actin filaments.
- Information by UniProt
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ab10149 has been referenced in 3 publications.
- Conte IL et al. Interaction between MyRIP and the actin cytoskeleton regulates Weibel-Palade body trafficking and exocytosis. J Cell Sci 129:592-603 (2016). PubMed: 26675235
- Song J et al. TLR4-mediated expulsion of bacteria from infected bladder epithelial cells. Proc Natl Acad Sci U S A 106:14966-71 (2009). IP ; Human . PubMed: 19706440
- Nightingale TD et al. Rab27a and MyRIP regulate the amount and multimeric state of VWF released from endothelial cells. Blood 113:5010-8 (2009). ICC/IF ; Human . PubMed: 19270261