Overview

  • Product name
    Anti-Myristic acid antibody
  • Description
    Rat polyclonal to Myristic acid
  • Host species
    Rat
  • Specificity
    This antibody is specific for conjugated Myristic acid, it does not recognize free Myristic acid.
  • Tested applications
    Suitable for: IHC-Frmore details
  • Immunogen

    Chemical/ Small Molecule corresponding to Myristic acid conjugated to Bovine Serum Albumin (BSA).

Properties

Applications

Our Abpromise guarantee covers the use of ab37027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/500 - 1/1000.

Target

  • Relevance
    Myristic acid, also called tetradecanoic acid, is a common saturated fatty acid found in dairy products. Covalent attachment of fatty acids to proteins is a common form of protein modification which has been shown to influence both structure and interaction with membranes. Myristoylation of proteins involves the NH(2)-terminal glycine residues forming an amide bond with the 14-carbon saturated acyl group. Myristic acid is also commonly added to a penultimate nitrogen terminus glycine in receptor-associated kinases to confer the membrane localisation of the enzyme. This is achieved by the myristic acid having a high enough hydrophobicity to become incorporated into the fatty acyl core of the phospholipid bilayer of the plasma membrane of the eukaryotic cell.
  • Alternative names
    • n Tetradecanoic acid antibody
    • Tetradecanoic acid antibody

References

ab37027 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer


In this case, as you had to wait long for our answer as well, I am willing to make an exception and provide you the testing discount. I do think indeed that your feedback would be beneficial also for other researchers and am therefore pleased that you would like to test ab37027 against the peptide conjugated myristic acid. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview with the results of the antibody against the peptide conjugated myristic acid and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting us.
The laboratory confirmed that unfortunately the binding capability of this antibody to myristic acid attached to a peptide has not been evaluated.
I can confirm also that the antibody will NOT recognize FREE Myristic acid, but it will recognize Myristic acid in the presence of gluteraldehyde. According to the lab, the gluteraldehyde does not have to be physically linked to the Myristic acid.
If you would like to test whether the ab37027 does react with Myristic acid conjugated to peptide, I can propose you our testing offer: I can offer a discount off a future purchase, if you buy ab37027 now, test it against the peptide conjugated Myristic acid and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.
If you are interested in this offer, please follow these steps:
1. Reply to this e-mail to let me know that you would like to proceed and test ab37027 against the peptide conjugated Myristic acid. I will then send a discount code. This code must be issued before purchasing ab37027 so please wait for my reply before ordering.
2. Purchase ab37027 either by phone, fax, or online (www.abcam.com).
3. Test it against the peptide conjugated Myristic acid.
4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab37027 turns out to be unsuitable for peptide conjugated Myristic acid, you will still receive the discount on your next purchase after your Abreview has been submitted.
Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting us. The concentration has not been determined for this product. If you would like to use it for IP, I would recommend starting with a 1/100 dilution and doing a titration to optimize. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

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Answer

Thank you for your enquiry. In this case, it is very possible that access to the epitope of ab37027 may be blocked by the presence of the conjugated antibody. Therefore, I am sorry we would not be able to guarantee that ab37027 would detect in this case without specifically testing. I am sorry this is not better news for you on this occasion. If you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for your enquiry. I can confirm that ab37027 Myristic acid antibody has not been tested for reactivity with 2-OH-Myristic acid. Testing has shown it will not recognize free myristic acid. The immunogen (Myristic acid-BSA) used to raise this antibody was BSA acylated with myristic acid. The fatty acid linkage is similar to that in cell membranes, so the antibody could recognize myristic acid on bacterial cell walls, but that hasn't been tested specifically. I hope this information is helpful to you. Should you have any further questions, please do not hesitate to contact us.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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