Key features and details
- Rabbit polyclonal to MYSM1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-MYSM1 antibody
See all MYSM1 primary antibodies
DescriptionRabbit polyclonal to MYSM1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- WB: U-87 MG, A431, HepG2, HeLa, Jurkat cell lysates; Mouse and rat brain tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab180570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 95 kDa.|
FunctionMetalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. Acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation.
Sequence similaritiesBelongs to the peptidase M67A family. MYSM1 subfamily.
Contains 1 MPN (JAB/Mov34) domain.
Contains 1 SANT domain.
Contains 1 SWIRM domain.
DomainBinds double-stranded DNA via the SANT domain. The SWIRM domain does not bind double-stranded DNA.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 2A-DUB antibody
- 2ADUB antibody
- Histone H2A deubiquitinase antibody
All lanes : Anti-MYSM1 antibody (ab180570) at 1/3000 dilution
Lane 1 : U-87 MG cell lysate
Lane 2 : A-431 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : Jurkat cell lysate
Lane 6 : Mouse brain tissue lysate
Lane 7 : Rat brain tissue lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat AntiRabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 95 kDa
Exposure time: 90 seconds
Blocking buffer: 3% nonfat dry milk in TBST
ab180570 has been referenced in 1 publication.
- Li Y et al. Expression of MYSM1 is associated with tumor progression in colorectal cancer. PLoS One 12:e0177235 (2017). WB . PubMed: 28498834