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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-MYSM1 antibody [EPR18657] (ab193081)

Reviews (1) Submit a question References (3)
Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
  • Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
  • Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
  • Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
  • Immunoprecipitation - Anti-MYSM1 antibody [EPR18657] (ab193081)
  • Anti-MYSM1 antibody [EPR18657] (ab193081)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18657] to MYSM1
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-MYSM1 antibody [EPR18657]
    See all MYSM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR18657] to MYSM1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK-293, HepG2, NCCIT, K562, RAW 264.7, PC-12, NIH/3T3 and mESC whole cell lysates. Wild-type HEK-293T cell lysate. IP: HeLa whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab193081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 66, 95 kDa (predicted molecular weight: 66, 95 kDa).
IP
1/100.
Notes
WB
1/1000. Detects a band of approximately 66, 95 kDa (predicted molecular weight: 66, 95 kDa).
IP
1/100.

Target

  • Function

    Metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. Acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation.

  • Sequence similarities

    Belongs to the peptidase M67A family. MYSM1 subfamily.
    Contains 1 MPN (JAB/Mov34) domain.
    Contains 1 SANT domain.
    Contains 1 SWIRM domain.

  • Domain

    Binds double-stranded DNA via the SANT domain. The SWIRM domain does not bind double-stranded DNA.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 2A-DUB antibody
    • 2ADUB antibody
    • Histone H2A deubiquitinase antibody
    • Histone H2A deubiquitinase MYSM1 antibody
    • KIAA1915 antibody
    • Myb like SWIRM and MPN domains 1 antibody
    • Myb like, SWIRM and MPN domain containing protein antibody
    • Myb like, SWIRM and MPN domains 1 antibody
    • Myb-like antibody
    • Myb-like, SWIRM and MPN domains 1 antibody
    • MYSM 1 antibody
    • mysm1 antibody
    • MYSM1 Myb-like, SWIRM and MPN domains 1 antibody
    • MYSM1_HUMAN antibody
    • RP4-592A1.1 antibody
    • SWIRM and MPN domain-containing protein 1 antibody
    see all

Images

  • Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
    Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
    All lanes : Anti-MYSM1 antibody [EPR18657] (ab193081) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : MYSM1 KO HEK-293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : RT-4 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 66, 95 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?



    False colour image of Western blot: Anti-MYSM1 antibody [EPR18657] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab193081 was shown to bind specifically to MYSM1. A band was observed at 110 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in MYSM1 knockout cell line ab267297 (knockout cell lysate ab257548). To generate this image, wild-type and MYSM1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

  • Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
    Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
    All lanes : Anti-MYSM1 antibody [EPR18657] (ab193081) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : NCCIT (Human pluripotent embryonic carcinoma cell line) whole cell lysate
    Lane 5 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 66, 95 kDa
    Observed band size: 66,95 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Two bands detected represent two isoforms.

  • Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
    Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
    Anti-MYSM1 antibody [EPR18657] (ab193081) at 1/1000 dilution + mESC (Mouse embryonic stem cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 66, 95 kDa
    Observed band size: 95 kDa why is the actual band size different from the predicted?


    Exposure time: 8 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
    Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
    All lanes : Anti-MYSM1 antibody [EPR18657] (ab193081) at 1/1000 dilution

    Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 66, 95 kDa
    Observed band size: 95 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • Immunoprecipitation - Anti-MYSM1 antibody [EPR18657] (ab193081)
    Immunoprecipitation - Anti-MYSM1 antibody [EPR18657] (ab193081)

    MYSM1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab193081 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab193081 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
    Lane 1: HeLa whole cell lysate 10µg (Input).
    Lane 2: ab193081 IP in HeLa whole cell lysate. 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab193081 in HeLa whole cell lysate.

    Predicted band size: 66kDa, 95kDa.

    Observed band size: 66kDa, 95kDa.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    Two bands detected represent two isoforms.

  • Anti-MYSM1 antibody [EPR18657] (ab193081)
    Anti-MYSM1 antibody [EPR18657] (ab193081)

References (3)

Publishing research using ab193081? Please let us know so that we can cite the reference in this datasheet.

ab193081 has been referenced in 3 publications.

  • Tian M  et al. MYSM1 Suppresses Cellular Senescence and the Aging Process to Prolong Lifespan. Adv Sci (Weinh) 7:2001950 (2020). PubMed: 33240758
  • Tian M  et al. MYSM1 Represses Innate Immunity and Autoimmunity through Suppressing the cGAS-STING Pathway. Cell Rep 33:108297 (2020). PubMed: 33086059
  • Panda S & Gekara NO The deubiquitinase MYSM1 dampens NOD2-mediated inflammation and tissue damage by inactivating the RIP2 complex. Nat Commun 9:4654 (2018). PubMed: 30405132

Customer reviews and Q&As

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Western blot abreview for Anti-MYSM1 antibody [EPR18657]

 Inconclusive
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HAP1 cell line)
Gel Running Conditions
Non-reduced Denaturing (gradient 4-12%)
Loading amount
50 µg
Specification
HAP1 cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Feb 21 2017

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