RecombinantRabMAb

Recombinant Anti-MYSM1 antibody [EPR18657] (ab193081)

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Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
  • Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
  • Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
  • Immunoprecipitation - Anti-MYSM1 antibody [EPR18657] (ab193081)
  • Anti-MYSM1 antibody [EPR18657] (ab193081)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18657] to MYSM1
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MYSM1 antibody [EPR18657]
    See all MYSM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR18657] to MYSM1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human MYSM1 aa 250-500. The exact sequence is proprietary.
    Database link: Q5VVJ2

  • Positive control

    • WB: HeLa, HEK-293, HepG2, NCCIT, K562, RAW 264.7, PC-12, NIH/3T3 and mESC whole cell lysates. IP: HeLa whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab193081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 66, 95 kDa (predicted molecular weight: 66, 95 kDa).
IP 1/100.

Target

  • Function

    Metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. Acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation.

  • Sequence similarities

    Belongs to the peptidase M67A family. MYSM1 subfamily.
    Contains 1 MPN (JAB/Mov34) domain.
    Contains 1 SANT domain.
    Contains 1 SWIRM domain.

  • Domain

    Binds double-stranded DNA via the SANT domain. The SWIRM domain does not bind double-stranded DNA.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 2A-DUB antibody
    • 2ADUB antibody
    • Histone H2A deubiquitinase antibody
    • Histone H2A deubiquitinase MYSM1 antibody
    • KIAA1915 antibody
    • Myb like SWIRM and MPN domains 1 antibody
    • Myb like, SWIRM and MPN domain containing protein antibody
    • Myb like, SWIRM and MPN domains 1 antibody
    • Myb-like antibody
    • Myb-like, SWIRM and MPN domains 1 antibody
    • MYSM 1 antibody
    • mysm1 antibody
    • MYSM1 Myb-like, SWIRM and MPN domains 1 antibody
    • MYSM1_HUMAN antibody
    • RP4-592A1.1 antibody
    • SWIRM and MPN domain-containing protein 1 antibody
    see all

Images

  • Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
    Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
    All lanes : Anti-MYSM1 antibody [EPR18657] (ab193081) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : NCCIT (Human pluripotent embryonic carcinoma cell line) whole cell lysate
    Lane 5 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 66, 95 kDa
    Observed band size: 66,95 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Two bands detected represent two isoforms.

  • Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
    Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
    Anti-MYSM1 antibody [EPR18657] (ab193081) at 1/1000 dilution + mESC (Mouse embryonic stem cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 66, 95 kDa
    Observed band size: 95 kDa why is the actual band size different from the predicted?


    Exposure time: 8 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
    Western blot - Anti-MYSM1 antibody [EPR18657] (ab193081)
    All lanes : Anti-MYSM1 antibody [EPR18657] (ab193081) at 1/1000 dilution

    Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 66, 95 kDa
    Observed band size: 95 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • Immunoprecipitation - Anti-MYSM1 antibody [EPR18657] (ab193081)
    Immunoprecipitation - Anti-MYSM1 antibody [EPR18657] (ab193081)

    MYSM1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab193081 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab193081 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
    Lane 1: HeLa whole cell lysate 10µg (Input).
    Lane 2: ab193081 IP in HeLa whole cell lysate. 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab193081 in HeLa whole cell lysate.

    Predicted band size: 66kDa, 95kDa.

    Observed band size: 66kDa, 95kDa.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    Two bands detected represent two isoforms.

  • Anti-MYSM1 antibody [EPR18657] (ab193081)
    Anti-MYSM1 antibody [EPR18657] (ab193081)

References (1)

Publishing research using ab193081? Please let us know so that we can cite the reference in this datasheet.

ab193081 has been referenced in 1 publication.

  • Panda S & Gekara NO The deubiquitinase MYSM1 dampens NOD2-mediated inflammation and tissue damage by inactivating the RIP2 complex. Nat Commun 9:4654 (2018). PubMed: 30405132

Customer reviews and Q&As

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Western blot abreview for Anti-MYSM1 antibody [EPR18657]

 Inconclusive
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HAP1 cell line)
Gel Running Conditions
Non-reduced Denaturing (gradient 4-12%)
Loading amount
50 µg
Specification
HAP1 cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Feb 21 2017

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