Anti-MyT1L antibody - C-terminal (ab139732)

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Immunocytochemistry/ Immunofluorescence - Anti-MyT1L antibody - C-terminal (ab139732)
  • Western blot - Anti-MyT1L antibody - C-terminal (ab139732)

Key features and details

  • Rabbit polyclonal to MyT1L - C-terminal
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-MyT1L antibody - C-terminal
    See all MyT1L primary antibodies

  • Description

    Rabbit polyclonal to MyT1L - C-terminal

  • Host species

    Rabbit

  • Specificity

    At least three isoforms are known to exist. ab139732 will only recognize the two longest isoforms.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to 18 amino acids near the C-terminus of Human MyT1L (NP_055840).

  • Positive control

    • Mouse Brain Tissue Lysate

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.

  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab139732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Mouse
Application Abreviews Notes
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 133 kDa.
ICC/IF
Use a concentration of 20 µg/ml.
Notes
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 133 kDa.
ICC/IF
Use a concentration of 20 µg/ml.

Target

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-MyT1L antibody - C-terminal (ab139732)
    Immunocytochemistry/ Immunofluorescence - Anti-MyT1L antibody - C-terminal (ab139732)
    Immunofluorescence of MyT1L in Human brain with ab139732 at 20 ug/mL.
  • Western blot - Anti-MyT1L antibody - C-terminal (ab139732)
    Western blot - Anti-MyT1L antibody - C-terminal (ab139732)
    All lanes : Anti-MyT1L antibody - C-terminal (ab139732) at 1 µg/ml

    Lane 1 : Mouse Brain Tissue Lysate
    Lane 2 : Mouse Brain Tissue Lysate with blocking peptide

    Predicted band size: 133 kDa

References (1)

Publishing research using ab139732? Please let us know so that we can cite the reference in this datasheet.

ab139732 has been referenced in 1 publication.

  • Hiramoto M  et al. A type 2 diabetes-associated SNP in KCNQ1 (rs163184) modulates the binding activity of the locus for Sp3 and Lsd1/Kdm1a, potentially affecting CDKN1C expression. Int J Mol Med 41:717-728 (2018). WB ; Rat . PubMed: 29207083

Customer reviews and Q&As

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Question

Inquiry: regarding product: ab139732 Dear, I am looking for a specific antibody for MYT1L to perform IP with. As the epitope is at the C-terminus of the protein, I was wondering if it is located in a zinc finger domain. There is a high homology with MYT1, so does the antibody pick up this protein too? How specific is the antibody? Is there cross reactivity with any other proteins? Is it possible to request a test sample? Thank you in advance. kind regards,

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Answer

Thank you for your enquiry and your interest in our products.

This antibody (ab139732: Rabbit polyclonal to MyT1L - C-terminal) is raised against a peptide from within the last 60 amino acids of NP_055840, therefore it is not from within a zinc finger domain.

A BLAST does not show any potential cross-reaction with other proteins aside from the other three MYT1L isoforms.

If you need any further assistance in the future, please do not hesitate to contact me.

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