Overview

  • Product name

  • Description

    Rabbit polyclonal to Myt1/MTF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Myt1/MTF1 conjugated to keyhole limpet haemocyanin.

  • Positive control

    • Induced culture of E.coli (one shot Top10 competent cells).
  • General notes

     This product was previously labelled as Myt1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab82844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: Use at an assay dependent dilution.
    Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/50000 is determined for one of the three peptides.
    WB: 1/1000. Detects a band of approximately 136 kDa (predicted molecular weight: 136 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Binds to the promoter regions of proteolipid proteins of the central nervous system. May play a role in the development of neurons and oligodendrogalia in the CNS. May regulate a critical transition point in oligodendrocyte lineage development by modulating oligodendrocyte progenitor proliferation relative to terminal differentiation and up-regulation of myelin gene transcription.
    • Tissue specificity

      Mostly in developing nervous system. Expressed in neural progenitors and oligodendrocyte lineage cells. More highly expressed in oligodendrocyte progenitors than in differentiated oligodendrocytes.
    • Sequence similarities

      Contains 7 C2HC-type zinc fingers.
    • Domain

      Contains 7 zinc fingers of the C2HC class arranged in two widely separated clusters. These two domains of DNA binding can function independently and recognize the same DNA sequence.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • C20orf36 antibody
      • KIAA0835 antibody
      • KIAA1050 antibody
      • MTF1 antibody
      • Myelin transcription factor 1 antibody
      • Myelin transcription factor I antibody
      • MYT1 antibody
      • MYT1_HUMAN antibody
      • MyTI antibody
      • PLPB1 antibody
      • Proteolipid protein-binding protein antibody
      see all

    Images

    • All lanes : Anti-Myt1/MTF1 antibody (ab82844) at 1/1000 dilution

      Lane 1 : uninduced culture of E.coli (one shot Top10 competent cells)
      Lane 2 : induced culture of E.coli (one shot Top10 competent cells)

      Secondary
      All lanes : anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size: 136 kDa
      Observed band size: 136 kDa
      Additional bands at: 35 kDa, 80 kDa. We are unsure as to the identity of these extra bands.



      Plasmid name: pBAD-DEST49.
      Predicted molecular weight is 122.3kDa, tag is 14kDa.

    References

    This product has been referenced in:

    • Fernandez-Enright F  et al. Novel implications of Lingo-1 and its signaling partners in schizophrenia. Transl Psychiatry 4:e348 (2014). WB ; Human . Read more (PubMed: 24448210) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab82844.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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