Overview

  • Product name

    Anti-MZF1 antibody
  • Description

    Rabbit polyclonal to MZF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human MZF1 (N terminal).

  • Positive control

    • Extracts from 293 and K562 cells; human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab64866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/10000.

Target

  • Function

    Binds to target promoter DNA and functions as trancription regulator. Regulates transcription from the PADI1 and CDH2 promoter. May be one regulator of transcriptional events during hemopoietic development.
  • Tissue specificity

    Preferentially expressed in differentiating myeloid cells. Detected in osteoblasts.
  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 13 C2H2-type zinc fingers.
    Contains 1 SCAN box domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Myeloid zinc finger 1 antibody
    • MZF 1 antibody
    • MZF-1 antibody
    • MZF1 antibody
    • MZF1_HUMAN antibody
    • MZF1B antibody
    • Zfp98 antibody
    • Zinc finger and SCAN domain containing protein 6 antibody
    • Zinc finger and SCAN domain-containing protein 6 antibody
    • Zinc finger protein 42 antibody
    • ZNF42 antibody
    • ZSCAN6 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab64866 at a dilution of 1/50 - 1/100. Left hand panel - no immunising peptide; right hand panel - immunising peptide.
  • All lanes : Anti-MZF1 antibody (ab64866) at 1/500 dilution

    Lane 1 : Extracts from 293 cells with no immunising peptide
    Lane 2 : Extracts from K562 cells with no immunising peptide
    Lane 3 : Extracts from K562 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 82 kDa
    Observed band size: 82 kDa

  • ICC/IF image of ab64866 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64866, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Tvingsholm SA  et al. Let-7 microRNA controls invasion-promoting lysosomal changes via the oncogenic transcription factor myeloid zinc finger-1. Oncogenesis 7:14 (2018). IHC-P ; Human . Read more (PubMed: 29396433) »
  • Lee JH  et al. Upregulation of SMAD4 by MZF1 inhibits migration of human gastric cancer cells. Int J Oncol 50:272-282 (2017). Read more (PubMed: 27922669) »
See all 3 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Gel Running Conditions
Reduced Denaturing (4-12 Bis tris)
Loading amount
20 µg
Specification
293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C

Abcam user community

Verified customer

Submitted May 14 2014

Answer

Thank you for your email.

I apologize as I have made an error in writing the amino acid number the correct one is from aa11 to 23; means the majority of the immunogen sequence is in RAPPEDEGPVMVK sequence of human MZF1. http://www.uniprot.org/uniprot/P28698

I hope this information will be helpful. Should you have any further question please do not hesitate to contact me.

Read More

Answer

Thank you for your phone call. I am very sorry to hear that you are having problems with this antibody. As discussed I have issued a free of charge replacement vial from new lot which will be shipped on 3rd January 2012. The order number is 1000786. I hope this will help. Should you have any question regarding this order please do not hesitate to contact me.

Read More
Abreviews
Application
ChIP
Sample
Human Cell lysate - nuclear (cervical tumor cell lines)
Negative control
primary keratinocytes
Specification
cervical tumor cell lines
Detection step
Semiquantitative PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Positive control
HeLa cells

Abcam user community

Verified customer

Submitted Jan 03 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (cervical tumor cell lines)
Specification
cervical tumor cell lines
Fixative
Paraformaldehyde
Permeabilization
Yes - triton
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 31 2010

Application
Western blot
Sample
Human Cell lysate - whole cell (human keratinocytes)
Loading amount
50 µg
Specification
human keratinocytes
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Nov 15 2010

Application
Western blot
Sample
Human Cell lysate - whole cell (different keratinocytes cell lines)
Loading amount
50 µg
Specification
different keratinocytes cell lines
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 11 2010

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