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Rabbit polyclonal to N acetylglucosamine kinase
Predicted to work with:
Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
A region between residue 50 and 100 of Human N acetylglucosamine kinase (NP_060037.2)
Whole cell lysate from HeLa cells, 293T cell lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration information loading...
Immunogen affinity purified
ab95182 was affinity purified using an epitope specific to NAGK immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 37 kDa.
Use at 2-5 µg/mg of lysate.
Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Also has ManNAc kinase activity.
Belongs to the eukaryotic-type N-acetylglucosamine kinase family.
Information by UniProt
EC 220.127.116.11 antibody
GlcNAc kinase antibody
Western blot - Anti-N acetylglucosamine kinase antibody (ab95182)
All lanes : Anti-N acetylglucosamine kinase antibody (ab95182) at 0.4 µg/ml Lane 1 : Whole cell lysate from HeLa at 50 µg Lane 2 : Whole cell lysate from HeLa at 15 µg Lane 3 : Whole cell lysate from HeLa at 5 µg Lane 4 : 293T cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 37 kDa Exposure time: 30 seconds
Immunoprecipitation - Anti-N acetylglucosamine kinase antibody (ab95182)
Detection of Human N acetylglucosamine kinase by Immunoprecipitation and Western Blot.
Whole cell lysate from HeLa at 1 mg for IP, 20% of IP loaded.
ab95182 was used for IP at 3 mcg/mg lysate and 1ug/ml for wb (lane 2)
N acetylglucosamine kinase was also immunoprecipitated by another rabbit antibody (lane 1) and control IgG (lane 3).
Detection: Chemiluminescence with exposure times of 30 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"