N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric) (ab204705)

Overview

  • Product name

    N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric)
  • Detection method

    Colorimetric
  • Sample type

    Urine, Serum, Tissue, Adherent cells, Suspension cells
  • Assay type

    Enzyme activity
  • Sensitivity

    50 µU
  • Assay time

    0h 25m
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric) (ab204705) provides a simple and sensitive method for monitoring NAG enzymatic activity. In this assay, NAG uses a synthetic p-nitrophenol derivative (R-pNP) as a NAG substrate and releases pNP which can be measured at absorbance (OD 400 nm). The assay can detect as low as 50 µU of NAG activity in a variety of samples.


    beta-NAG assay protocol summary:
    - add samples and standards to wells
    - add NAG substrate and incubate for 5-30 min
    - add stop solution and incubate for 10 min
    - analyze with microplate reader

  • Notes

    β-N-Acetylglucosaminidase (NAG, EC 3.2.1.52) is a lysosomal enzyme that is expressed in various tissues, including kidney, liver and lungs. NAG can cleave N-acetyl-glucosamine, a monosaccharide derivative of glucose. Its concentration in urine is minimal due to its inability to cross the glomerular basal membrane. Increased concentration of NAG in urine indicates renal tubular cell breakdown. Acute Kidney Injury (AKI) is the sudden loss of kidney functions, causing electrolyte imbalance, and retention of urea and other nitrogenous products. NAG has become one of the most studied and used biomarkers for the detection and diagnosis of AKI.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    NAG Assay Buffer 1 x 35ml
    NAG Positive Control 1 vial
    NAG Stop Solution 1 x 3ml
    NAG Substrate 1 x 6ml
    p-Nitrophenol (pNP) (20 mM) 1 x 100µl

Images

  • Typical pNP Standard calibration curve.

  • Measurement of NAG activity in human urine from different donors. Undiluted samples (70 µL) were incubated for 30 min. with NAG substrate.

  • Measurement of NAG activity in mouse kidney (10 µg) and human serum (20 µL). Samples were incubated for 30 min. with NAG substrate.

Protocols

References

This product has been referenced in:

  • Siddiqui K  et al. Urinary N-acetyl-beta-d-glucosaminidase (NAG) with neutrophil gelatinase-associated lipocalin (NGAL) improves the diagnostic value for proximal tubule damage in diabetic kidney disease. 3 Biotech 9:66 (2019). Read more (PubMed: 30729090) »
  • de Groot T  et al. Lithium reduces blood glucose levels, but aggravates albuminuria in BTBR-ob/ob mice. PLoS One 12:e0189485 (2017). Read more (PubMed: 29244860) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab204705.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up