Overview

  • Product name
    Anti-N Cadherin antibody [5D5]
    See all N Cadherin primary antibodies
  • Description
    Mouse monoclonal [5D5] to N Cadherin
  • Host species
    Mouse
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, WB, IHC-P, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human N Cadherin.

  • Positive control
    • A431, NIH-3T3, Hela, C6 and LNCap cells. Human lung cancer, colon cancer, ovarian cancer and mammary cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab98952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 100 kDa.
IHC-P 1/200 - 1/1000.
ICC/IF 1/200 - 1/1000.
ELISA 1/10000.

Target

  • Function
    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
  • Sequence similarities
    Contains 5 cadherin domains.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CADH2_HUMAN antibody
    • Cadherin 2 antibody
    • Cadherin 2 N cadherin neuronal antibody
    • Cadherin 2 type 1 antibody
    • Cadherin 2 type 1 N cadherin neuronal antibody
    • Cadherin 2, type 1, N-cadherin (neuronal) antibody
    • Cadherin-2 antibody
    • Cadherin2 antibody
    • Calcium dependent adhesion protein neuronal antibody
    • CD325 antibody
    • CD325 antigen antibody
    • CDH2 antibody
    • CDHN antibody
    • CDw325 antibody
    • CDw325 antigen antibody
    • N cadherin 1 antibody
    • N-cadherin antibody
    • NCAD antibody
    • Neural cadherin antibody
    • OTTHUMP00000066304 antibody
    • OTTHUMP00000067378 antibody
    see all

Images

  • All lanes : Anti-N Cadherin antibody [5D5] (ab98952) at 1/500 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : NIH-3T3 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : C6 cell lysate
    Lane 5 : LNCap cell lysate

    Predicted band size: 100 kDa

  • ab98952 staining N Cadherin in human NSCLC tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with using HOPE technique and blocked for 5 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab98952 staining N Cadherin in paraffin-embedded human lung cancer tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with HOPE and blocked. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. A TRITC-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab98952 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab98952, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunohistochemical analysis of paraffin-embedded Human lung cancer (1), colon cancer (2), ovarian cancer (3) and mammary cancer (4), using ab98952 at 1/200 dilution with DAB staining.
  • Immunofluorescence analysis of A431 cells using ab98952 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye
  • ab98952 staining N Cadherin (orange) in Mouse E15.5 Brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/200 in PBST) for 12 hours at 4°C. An Alexa Fluor® 568-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Liao Z  et al. High PLK4 expression promotes tumor progression and induces epithelial-mesenchymal transition by regulating the Wnt/ß-catenin signaling pathway in colorectal cancer. Int J Oncol 54:479-490 (2019). Read more (PubMed: 30570110) »
  • Tang H  et al. MARCH5 overexpression contributes to tumor growth and metastasis and associates with poor survival in breast cancer. Cancer Manag Res 11:201-215 (2019). Read more (PubMed: 30636894) »
See all 46 Publications for this product

Customer reviews and Q&As

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1-5 of 5 Abreviews

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (embryonic mouse brain coronal cortical section)
Permeabilization
Yes - Triton X
Specification
embryonic mouse brain coronal cortical section
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Dr. Bhavin Shah

Verified customer

Submitted Oct 20 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
ZytoChem-Plus HRP Polymer-Kit Blocking solution as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Antigen retrieval step
None
Sample
Human Tissue sections (NSCLC)
Specification
NSCLC
Permeabilization
No
Fixative
HOPE

Abcam user community

Verified customer

Submitted Apr 13 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Zytomed Systems HRP Polymer Blocking solution as blocking agent for 5 minute(s) · Concentration: 100%
Sample
Human Cell (paraffin-embedded lung cancer tissue)
Specification
paraffin-embedded lung cancer tissue
Permeabilization
No
Fixative
HOPE

Abcam user community

Verified customer

Submitted Mar 18 2014

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E15.5 brain)
Specification
E15.5 brain
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Dr. Nam-Ho Kim

Verified customer

Submitted May 21 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (EpiSCs)
Specification
EpiSCs
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton 0.1%
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Nov 09 2011

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