Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952)
Key features and details
- Mouse monoclonal [5D5] to N Cadherin - Intercellular Junction Marker
- Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker
See all N Cadherin primary antibodies -
Description
Mouse monoclonal [5D5] to N Cadherin - Intercellular Junction Marker -
Host species
Mouse -
Tested Applications & Species
Application Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human -
Immunogen
Recombinant fragment corresponding to Human N Cadherin.
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Positive control
- A431, NIH-3T3, Hela, C6 and LNCap cells. Human lung cancer, colon cancer, ovarian cancer and mammary cancer tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
5D5 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab98952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Human
|
ICC/IF |
Human
|
IHC-P |
Human
|
WB |
Human
|
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
WB |
1/500 - 1/2000. Predicted molecular weight: 100 kDa.
|
|
IHC-P | (1) |
1/200 - 1/1000.
|
ICC/IF | (2) |
1/200 - 1/1000.
|
Notes |
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Flow Cyt
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/500 - 1/2000. Predicted molecular weight: 100 kDa. |
IHC-P
1/200 - 1/1000. |
ICC/IF
1/200 - 1/1000. |
Target
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Function
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. -
Sequence similarities
Contains 5 cadherin domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1000 Human
- Entrez Gene: 12558 Mouse
- Entrez Gene: 83501 Rat
- Omim: 114020 Human
- SwissProt: P19022 Human
- SwissProt: P15116 Mouse
- SwissProt: Q9Z1Y3 Rat
- Unigene: 464829 Human
see all -
Alternative names
- CADH2_HUMAN antibody
- Cadherin 2 antibody
- Cadherin 2 N cadherin neuronal antibody
see all
Images
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All lanes : Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952) at 1/500 dilution
Lane 1 : A431 cell lysate
Lane 2 : NIH-3T3 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : C6 cell lysate
Lane 5 : LNCap cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : HRP-Goat Anti-Mouse Igg (Fc) at 1/10000 dilution
Predicted band size: 100 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952)This image is courtesy of an anonymous Abreview
ab98952 staining N Cadherin in human NSCLC tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with using HOPE technique and blocked for 5 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952)This image is courtesy of an anonymous Abreview
ab98952 staining N Cadherin in paraffin-embedded human lung cancer tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with HOPE and blocked. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. A TRITC-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
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Overlay histogram showing HeLa cells stained with ab98952 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab98952, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952)Immunohistochemical analysis of paraffin-embedded Human lung cancer (1), colon cancer (2), ovarian cancer (3) and mammary cancer (4), using ab98952 at 1/200 dilution with DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952)Immunofluorescence analysis of A431 cells using ab98952 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye
Protocols
References (78)
ab98952 has been referenced in 78 publications.
- Chow T et al. Recapitulating kidney development in vitro by priming and differentiating mouse embryonic stem cells in monolayers. NPJ Regen Med 5:7 (2020). PubMed: 32351711
- Zheng Y et al. Altered expression of glycoprotein non-metastatic melanoma protein B in the distal sciatic nerve following injury. Int J Mol Med 45:1909-1917 (2020). PubMed: 32236569
- Tan S et al. RAC1 Involves in the Radioresistance by Mediating Epithelial-Mesenchymal Transition in Lung Cancer. Front Oncol 10:649 (2020). PubMed: 32411607
- Tiwari R et al. Androgen deprivation upregulates SPINK1 expression and potentiates cellular plasticity in prostate cancer. Nat Commun 11:384 (2020). PubMed: 31959826
- Yang L et al. SP1 induced long non-coding RNA LINC00958 overexpression facilitate cell proliferation, migration and invasion in lung adenocarcinoma via mediating miR-625-5p/CPSF7 axis. Cancer Cell Int 20:24 (2020). PubMed: 31997940