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I have bought your Abcam (ab18203) rabbit policlonal anti-cadherin antibody and used based on the information on your site "Predicted to work with cow". I have used it on my bovine samples and it works fine, at a 1/50 dilution. However, my non-selective IgG negative control also staining green! Could you please specify if it was tested in bovine or if any publications has done it and which negative control was used? Best wishes.
Asked on Jan 26 2012
Thank you for contacting Abcam.
I am glad to hear that the antibody is working well in bovine tissues. I was wondering if you would be able to provide me with some additional information about your negative controls, is control IgG an Abcam product? Does it match the species, isotype, clonality and conjugation of ab18203. Have you considered using the blocking peptide that is available as a negative control (ab18620; https://www.abcam.com/n-cadherin-peptide-ab18620.html)?
Ab18203 is not tested by us for specific reaction with bovine tissue. The "predicted to work" classification is based on homology between the immunogen and the bovine protein.
Please let me know if you have any questions.
Answered on Jan 26 2012