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Dear Sir or Madam
I just wondered whether you are able to provide some information about the antibody anti-n-cadherin catalogue number ab18203 please? The main detail that I require is whether this antibody binds to the extracellular or intracellular part of the N-cadherin molecule? I.e. if it’s intracellular I’d have to permeablise the cells but if it’s extracellular then I wouldn’t need to permeablise the cells.
Thanks for your time and I look forward to hearing from you
Asked on Aug 02 2012
Thank you for your inquiry.
I can confirm that the immunogen is a synthetic peptide conjugated to KLH derived from within residues 800 - 900 of Human N Cadherin.
According to the UniProt homepage (http://www.uniprot.org/uniprot/P19022) amino acids 746-906 are cytoplasmic.
I can therefore confirm that a permeabilisation will be necessary for your experiments.
I wish you good luck and hope this information was helpful.
Answered on Aug 02 2012