Anti-N Cadherin antibody (ab211126)
Key features and details
- Rabbit polyclonal to N Cadherin
- Suitable for: WB, IHC - Wholemount
- Reacts with: Zebrafish
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-N Cadherin antibody
See all N Cadherin primary antibodies -
Description
Rabbit polyclonal to N Cadherin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC - Wholemountmore details -
Species reactivity
Reacts with: Zebrafish -
Immunogen
Recombinant fragment within Zebrafish N Cadherin aa 117-304. The exact sequence is proprietary.
Database link: Q90275 -
Positive control
- Zebrafish brain whole cell lysate; Zebrafish embryo.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab211126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 99 kDa.
|
|
IHC - Wholemount |
1/50 - 1/500.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 99 kDa. |
IHC - Wholemount
1/50 - 1/500. |
Target
-
Function
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. -
Sequence similarities
Contains 5 cadherin domains. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 30291 Zebrafish
- SwissProt: Q90275 Zebrafish
- Unigene: 75081 Zebrafish
-
Alternative names
- CADH2_HUMAN antibody
- Cadherin 2 antibody
- Cadherin 2 N cadherin neuronal antibody
see all
Images
-
Immunohistochemical analysis (wholemount) of 2 days-post-fertilization zebrafish embryo labeling N Cadherin with ab211126 at 1/50 dilution.
-
Immunohistochemical analysis (wholemount) of 2 days-post-fertilization zebrafish embryo labeling N Cadherin with ab211126 at 1/50 dilution.
-
Anti-N Cadherin antibody (ab211126) at 1/1000 dilution + Zebrafish brain whole cell lysate at 30 µg
Predicted band size: 99 kDa7.5% SDS PAGE
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab211126 has been referenced in 4 publications.
- Gong Z et al. Downregulation of lncRNA NEAT1 inhibits the proliferation of human cutaneous squamous cell carcinoma in vivo and in vitro. Ann Transl Med 10:79 (2022). PubMed: 35282061
- Harding P et al. EPHA2 Segregates with Microphthalmia and Congenital Cataracts in Two Unrelated Families. Int J Mol Sci 22:N/A (2021). PubMed: 33671840
- Balaraju AK et al. Glypican 4 regulates planar cell polarity of endoderm cells by controlling the localization of Cadherin 2. Development 148:N/A (2021). PubMed: 34131730
- Klaver EJ et al. Protease-dependent defects in N-cadherin processing drive PMM2-CDG pathogenesis. JCI Insight 6:N/A (2021). PubMed: 34784297