Overview

  • Product name

    Anti-N Cadherin antibody [EPR19654]
    See all N Cadherin primary antibodies
  • Description

    Rabbit monoclonal [EPR19654] to N Cadherin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human N Cadherin aa 250-500. The exact sequence is proprietary.
    Database link: P19022

  • Positive control

    • WB: HepG2 and PC-3 whole cell lysates; human fetal brain, cerebellum and fetal liver lysates. IHC-P: Human cardiac muscle and endometrium tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab207608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 130, 110 kDa (predicted molecular weight: 100 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
  • Sequence similarities

    Contains 5 cadherin domains.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CADH2_HUMAN antibody
    • Cadherin 2 antibody
    • Cadherin 2 N cadherin neuronal antibody
    • Cadherin 2 type 1 antibody
    • Cadherin 2 type 1 N cadherin neuronal antibody
    • Cadherin 2, type 1, N-cadherin (neuronal) antibody
    • Cadherin-2 antibody
    • Cadherin2 antibody
    • Calcium dependent adhesion protein neuronal antibody
    • CD325 antibody
    • CD325 antigen antibody
    • CDH2 antibody
    • CDHN antibody
    • CDw325 antibody
    • CDw325 antigen antibody
    • N cadherin 1 antibody
    • N-cadherin antibody
    • NCAD antibody
    • Neural cadherin antibody
    • OTTHUMP00000066304 antibody
    • OTTHUMP00000067378 antibody
    see all

Images

  • All lanes : Anti-N Cadherin antibody [EPR19654] (ab207608) at 1/1000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
    Lane 3 : Human fetal brain lysate
    Lane 4 : Human cerebellum lysate
    Lane 5 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 3-5 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 100 kDa
    Observed band size: 110,130 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/2: 30 seconds; Lane 3: 5 seconds; Lane 4/5: 3 minutes.

    The molecular weight observed is consistent with what has been described in the literature(PMID: 22553038).

  • Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling N Cadherin with ab207608 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Intercalated disc staining on human cardiac muscle is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • All lanes : ab76011, Anti-N Cadherin antibody [EPR1791-4] (Left) or ab207608 (Right) at 1/1000 dilution

    Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method with 5% NFDM/TBST
    Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method with 5% NFDM/TBST
    Lane 3 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method with 5% NFDM/TBST
    Lane 4 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method with 5% NFDM/TBST
    Lane 5 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate prepared in RIPA lysis method with 5% NFDM/TBST
    Lane 6 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate prepared in 1%SDS Hot lysis method with 5% NFDM/TBST
    Lane 7 : C6 (Rat glial tumor glial cell) whole cell lysates prepared in RIPA lysis method with 5% NFDM/TBST
    Lane 8 : C6 (Rat glial tumor glial cell) whole cell lysates prepared in 1%SDS Hot lysis method with 5% NFDM/TBST
    Lane 9 : Human brain lysates prepared in 1%SDS Hot lysis method with 5% NFDM/TBST
    Lane 10 : Mouse brain lysates prepared in 1%SDS Hot lysis method with 5% NFDM/TBST
    Lane 11 : Rat brain lysates prepared in 1%SDS Hot lysis method with 5% NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 100 kDa
    Observed band size: 110-130 kDa why is the actual band size different from the predicted?



    Exposure time:

    Lane 1 and 2: 4 seconds
    Lane 4 to 11: 1 seconds

    The molecular weight observed is consistent with what has been described in the literature (PMID: 22553038). This antibody fails to detect N Cadherin in HCT 116 cell which is positive described in the literature (PMID: 23431386 and 26540342)

  • Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling N Cadherin with ab207608 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Membrane staining on human endometrium is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Devarasetty M  et al. Bioengineered Submucosal Organoids for In Vitro Modeling of Colorectal Cancer. Tissue Eng Part A 23:1026-1041 (2017). Read more (PubMed: 28922975) »
See 1 Publication for this product

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