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    n-cadherin-antibody-epr19654-ab207608.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins
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RecombinantRabMAb

Recombinant Anti-N Cadherin antibody [EPR19654] (ab207608)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (8)

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Western blot - Anti-N Cadherin antibody [EPR19654] (ab207608)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR19654] (ab207608)
  • Western blot - Anti-N Cadherin antibody [EPR19654] (ab207608)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR19654] (ab207608)
  • Anti-N Cadherin antibody [EPR19654] (ab207608)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19654] to N Cadherin
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
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Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030)
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Human CDH2 (N Cadherin) knockout HEK-293T cell line (ab255377)

View more associated products

Overview

  • Product name

    Anti-N Cadherin antibody [EPR19654]
    See all N Cadherin primary antibodies
  • Description

    Rabbit monoclonal [EPR19654] to N Cadherin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2 and PC-3 whole cell lysates; human fetal brain, cerebellum and fetal liver lysates. IHC-P: Human cardiac muscle and endometrium tissues.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19654
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Cadherins

Associated products

  • Alternative Versions

    • Anti-N Cadherin antibody [EPR19654] - BSA and Azide free (ab236032)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab207608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 130, 110 kDa (predicted molecular weight: 100 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000. Detects a band of approximately 130, 110 kDa (predicted molecular weight: 100 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
  • Sequence similarities

    Contains 5 cadherin domains.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P19022 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1000 Human
    • Entrez Gene: 12558 Mouse
    • Entrez Gene: 83501 Rat
    • Omim: 114020 Human
    • SwissProt: P19022 Human
    • SwissProt: P15116 Mouse
    • SwissProt: Q9Z1Y3 Rat
    • Unigene: 464829 Human
    • Unigene: 606106 Human
    • Unigene: 257437 Mouse
    • Unigene: 23200 Rat
    see all
  • Alternative names

    • CADH2_HUMAN antibody
    • Cadherin 2 antibody
    • Cadherin 2 N cadherin neuronal antibody
    • Cadherin 2 type 1 antibody
    • Cadherin 2 type 1 N cadherin neuronal antibody
    • Cadherin 2, type 1, N-cadherin (neuronal) antibody
    • Cadherin-2 antibody
    • Cadherin2 antibody
    • Calcium dependent adhesion protein neuronal antibody
    • CD325 antibody
    • CD325 antigen antibody
    • CDH2 antibody
    • CDHN antibody
    • CDw325 antibody
    • CDw325 antigen antibody
    • N cadherin 1 antibody
    • N-cadherin antibody
    • NCAD antibody
    • Neural cadherin antibody
    • OTTHUMP00000066304 antibody
    • OTTHUMP00000067378 antibody
    see all

Images

  • Western blot - Anti-N Cadherin antibody [EPR19654] (ab207608)
    Western blot - Anti-N Cadherin antibody [EPR19654] (ab207608)
    All lanes : Anti-N Cadherin antibody [EPR19654] (ab207608) at 1/1000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
    Lane 3 : Human fetal brain lysate
    Lane 4 : Human cerebellum lysate
    Lane 5 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 3-5 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 100 kDa
    Observed band size: 110,130 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/2: 30 seconds; Lane 3: 5 seconds; Lane 4/5: 3 minutes.

    The molecular weight observed is consistent with what has been described in the literature(PMID: 22553038).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR19654] (ab207608)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR19654] (ab207608)

    Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling N Cadherin with ab207608 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Intercalated disc staining on human cardiac muscle is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-N Cadherin antibody [EPR19654] (ab207608)
    Western blot - Anti-N Cadherin antibody [EPR19654] (ab207608)
    All lanes : ab76011, Anti-N Cadherin antibody [EPR1791-4] (Left) or ab207608 (Right) at 1/1000 dilution

    Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method with 5% NFDM/TBST
    Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method with 5% NFDM/TBST
    Lane 3 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method with 5% NFDM/TBST
    Lane 4 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method with 5% NFDM/TBST
    Lane 5 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate prepared in RIPA lysis method with 5% NFDM/TBST
    Lane 6 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate prepared in 1%SDS Hot lysis method with 5% NFDM/TBST
    Lane 7 : C6 (Rat glial tumor glial cell) whole cell lysates prepared in RIPA lysis method with 5% NFDM/TBST
    Lane 8 : C6 (Rat glial tumor glial cell) whole cell lysates prepared in 1%SDS Hot lysis method with 5% NFDM/TBST
    Lane 9 : Human brain lysates prepared in 1%SDS Hot lysis method with 5% NFDM/TBST
    Lane 10 : Mouse brain lysates prepared in 1%SDS Hot lysis method with 5% NFDM/TBST
    Lane 11 : Rat brain lysates prepared in 1%SDS Hot lysis method with 5% NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 100 kDa
    Observed band size: 110-130 kDa why is the actual band size different from the predicted?



    Exposure time:

    Lane 1 and 2: 4 seconds
    Lane 4 to 11: 1 seconds

    The molecular weight observed is consistent with what has been described in the literature (PMID: 22553038). This antibody fails to detect N Cadherin in HCT 116 cell which is positive described in the literature (PMID: 23431386 and 26540342)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR19654] (ab207608)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR19654] (ab207608)

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling N Cadherin with ab207608 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Membrane staining on human endometrium is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-N Cadherin antibody [EPR19654] (ab207608)
    Anti-N Cadherin antibody [EPR19654] (ab207608)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (8)

Publishing research using ab207608? Please let us know so that we can cite the reference in this datasheet.

ab207608 has been referenced in 8 publications.

  • Wang H  et al. MicroRNA-15a-5p inhibits endometrial carcinoma proliferation, invasion and migration via downregulation of VEGFA and inhibition of the Wnt/ß-catenin signaling pathway. Oncol Lett 21:310 (2021). PubMed: 33732386
  • Ren R  et al. ZFPM2-AS1 transcriptionally mediated by STAT1 regulates thyroid cancer cell growth, migration and invasion via miR-515-5p/TUSC3. J Cancer 12:3393-3406 (2021). PubMed: 33976749
  • Yang Y  et al. SOX2-Upregulated microRNA-30e Promotes the Progression of Esophageal Cancer via Regulation of the USP4/SMAD4/CK2 Axis. Mol Ther Nucleic Acids 23:200-214 (2021). PubMed: 33376627
  • Chen S  et al. CLK1/SRSF5 pathway induces aberrant exon skipping of METTL14 and Cyclin L2 and promotes growth and metastasis of pancreatic cancer. J Hematol Oncol 14:60 (2021). PubMed: 33849617
  • Abrahamsson A  et al. Fulvestrant-Mediated Attenuation of the Innate Immune Response Decreases ER+ Breast Cancer Growth In Vivo More Effectively than Tamoxifen. Cancer Res 80:4487-4499 (2020). PubMed: 32855207
  • Long M  et al. Lysine-Specific Demethylase 1 Affects the Progression of Papillary Thyroid Carcinoma via HIF1a and microRNA-146a. J Clin Endocrinol Metab 105:N/A (2020). PubMed: 32303750
  • Guo X & Wang Y LncRNA TMPO-AS1 promotes hepatocellular carcinoma cell proliferation, migration and invasion through sponging miR-329-3p to stimulate FOXK1-mediated AKT/mTOR signaling pathway. Cancer Med 9:5235-5246 (2020). PubMed: 32462698
  • Devarasetty M  et al. Bioengineered Submucosal Organoids for In Vitro Modeling of Colorectal Cancer. Tissue Eng Part A 23:1026-1041 (2017). PubMed: 28922975

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