Recombinant
RabMAb

Recombinant Anti-N Cadherin antibody [EPR19654] (ab207608)

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Overview

  • Product name

    Anti-N Cadherin antibody [EPR19654]
    See all N Cadherin primary antibodies

  • Description

    Rabbit monoclonal [EPR19654] to N Cadherin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human N Cadherin aa 250-500. The exact sequence is proprietary.
    Database link: P19022

  • Positive control

    • WB: HepG2 and PC-3 whole cell lysates; human fetal brain, cerebellum and fetal liver lysates. IHC-P: Human cardiac muscle and endometrium tissues.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab207608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 130, 110 kDa (predicted molecular weight: 100 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.

  • Sequence similarities

    Contains 5 cadherin domains.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CADH2_HUMAN antibody
      • Cadherin 2 antibody
      • Cadherin 2 N cadherin neuronal antibody
      • Cadherin 2 type 1 antibody
      • Cadherin 2 type 1 N cadherin neuronal antibody
      • Cadherin 2, type 1, N-cadherin (neuronal) antibody
      • Cadherin-2 antibody
      • Cadherin2 antibody
      • Calcium dependent adhesion protein neuronal antibody
      • CD325 antibody
      • CD325 antigen antibody
      • CDH2 antibody
      • CDHN antibody
      • CDw325 antibody
      • CDw325 antigen antibody
      • N cadherin 1 antibody
      • N-cadherin antibody
      • NCAD antibody
      • Neural cadherin antibody
      • OTTHUMP00000066304 antibody
      • OTTHUMP00000067378 antibody
      see all

    Images

    • Western blot - Anti-N Cadherin antibody [EPR19654] (ab207608)
      Western blot - Anti-N Cadherin antibody [EPR19654] (ab207608)
      All lanes : Anti-N Cadherin antibody [EPR19654] (ab207608) at 1/1000 dilution

      Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 2 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
      Lane 3 : Human fetal brain lysate
      Lane 4 : Human cerebellum lysate
      Lane 5 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
      Lanes 3-5 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

      Predicted band size: 100 kDa
      Observed band size: 110,130 kDa
      why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1/2: 30 seconds; Lane 3: 5 seconds; Lane 4/5: 3 minutes.

      The molecular weight observed is consistent with what has been described in the literature(PMID: 22553038).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR19654] (ab207608)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR19654] (ab207608)

      Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling N Cadherin with ab207608 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Intercalated disc staining on human cardiac muscle is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-N Cadherin antibody [EPR19654] (ab207608)
      Western blot - Anti-N Cadherin antibody [EPR19654] (ab207608)
      All lanes : ab76011, Anti-N Cadherin antibody [EPR1791-4] (Left) or ab207608 (Right) at 1/1000 dilution

      Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method with 5% NFDM/TBST
      Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method with 5% NFDM/TBST
      Lane 3 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method with 5% NFDM/TBST
      Lane 4 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method with 5% NFDM/TBST
      Lane 5 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate prepared in RIPA lysis method with 5% NFDM/TBST
      Lane 6 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate prepared in 1%SDS Hot lysis method with 5% NFDM/TBST
      Lane 7 : C6 (Rat glial tumor glial cell) whole cell lysates prepared in RIPA lysis method with 5% NFDM/TBST
      Lane 8 : C6 (Rat glial tumor glial cell) whole cell lysates prepared in 1%SDS Hot lysis method with 5% NFDM/TBST
      Lane 9 : Human brain lysates prepared in 1%SDS Hot lysis method with 5% NFDM/TBST
      Lane 10 : Mouse brain lysates prepared in 1%SDS Hot lysis method with 5% NFDM/TBST
      Lane 11 : Rat brain lysates prepared in 1%SDS Hot lysis method with 5% NFDM/TBST

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 100 kDa
      Observed band size: 110-130 kDa why is the actual band size different from the predicted?



      Exposure time:

      Lane 1 and 2: 4 seconds
      Lane 4 to 11: 1 seconds

      The molecular weight observed is consistent with what has been described in the literature (PMID: 22553038). This antibody fails to detect N Cadherin in HCT 116 cell which is positive described in the literature (PMID: 23431386 and 26540342)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR19654] (ab207608)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR19654] (ab207608)

      Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling N Cadherin with ab207608 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Membrane staining on human endometrium is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Devarasetty M  et al. Bioengineered Submucosal Organoids for In Vitro Modeling of Colorectal Cancer. Tissue Eng Part A 23:1026-1041 (2017). Read more (PubMed: 28922975) »
    See 1 Publication for this product

    Publishing research using ab207608? Please let us know so that we can cite the reference in this datasheet.

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