Recombinant Anti-N Cadherin antibody [EPR22397-264] - BSA and Azide free (ab245827)
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Overview
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Product name
Anti-N Cadherin antibody [EPR22397-264] - BSA and Azide free
See all N Cadherin primary antibodies -
Description
Rabbit monoclonal [EPR22397-264] to N Cadherin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human N Cadherin aa 150-750. The exact sequence is proprietary.
Database link: P19022 -
Positive control
- WB: HeLa, PC-3, C6, A549 and HepG2 whole cell lysate. Human brain lysate. Mouse brain and heart lysate. Rat brain, heart and liver lysate. Flow Cyt: MCF7 cells. IP: N Cadherin IP in HeLa whole cell lysate.
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General notes
Ab245827 is the carrier-free version of ab245117. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab245827 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22397-264 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab245827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 130, 110 kDa (predicted molecular weight: 100 kDa). | |
| Flow Cyt | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. |
Target
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Function
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. -
Sequence similarities
Contains 5 cadherin domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1000 Human
- Entrez Gene: 12558 Mouse
- Entrez Gene: 83501 Rat
- Omim: 114020 Human
- SwissProt: P19022 Human
- SwissProt: P15116 Mouse
- SwissProt: Q9Z1Y3 Rat
- Unigene: 464829 Human
see all -
Alternative names
- CADH2_HUMAN antibody
- Cadherin 2 antibody
- Cadherin 2 N cadherin neuronal antibody
see all
Images
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![Flow Cytometry - Anti-N Cadherin antibody [EPR22397-264] - BSA and Azide free (ab245827)](https://www.abcam.com/ps/products/245/ab245827/Images/ab245827-1-245117FC1.jpg)
Flow Cytometry - Anti-N Cadherin antibody [EPR22397-264] - BSA and Azide free (ab245827)Flow cytometric analysis of MCF7 (Human breast adenocarcinoma epithelial cell, Left) / HeLa (Human cervix adenocarcinoma epithelial cell, Right) cell lines labeling N Cadherin with ab245117 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Negative control: MCF7 (PMID: 9177902).
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245117). -
![Immunoprecipitation - Anti-N Cadherin antibody [EPR22397-264] - BSA and Azide free (ab245827)](https://www.abcam.com/ps/products/245/ab245827/Images/ab245827-2-245117IP1.jpg)
Immunoprecipitation - Anti-N Cadherin antibody [EPR22397-264] - BSA and Azide free (ab245827)N Cadherin was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell line) whole cell lysate with ab245117 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab245117 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 10 µg (Input).
Lane 2: ab245117 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245117 in HeLa whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 seconds.The molecular weight is consistent with literature (PMID: 8230319).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245117).
Datasheets and documents
References
ab245827 has not yet been referenced specifically in any publications.
