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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins
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Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)

  • Datasheet
  • SDS
Reviews (47)Q&A (6)References (327)

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Western blot - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)
  • Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)
  • Immunoprecipitation - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)
  • Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)

Key features and details

  • Rabbit polyclonal to N Cadherin - Intercellular Junction Marker
  • Suitable for: WB, IHC-P, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-N Cadherin antibody - Intercellular Junction Marker
    See all N Cadherin primary antibodies
  • Description

    Rabbit polyclonal to N Cadherin - Intercellular Junction Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Pig, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Human N Cadherin aa 800-900 (internal sequence) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab18620)

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Cadherins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human N Cadherin protein (His tag) (ab223009)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Applications

Our Abpromise guarantee covers the use of ab18203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 125-135 kDa (predicted molecular weight: 100 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
  • Sequence similarities

    Contains 5 cadherin domains.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P19022 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 414745 Chicken
    • Entrez Gene: 281062 Cow
    • Entrez Gene: 1000 Human
    • Entrez Gene: 12558 Mouse
    • Entrez Gene: 83501 Rat
    • Omim: 114020 Human
    • SwissProt: P10288 Chicken
    • SwissProt: P19022 Human
    • SwissProt: P15116 Mouse
    • SwissProt: Q9Z1Y3 Rat
    • Unigene: 464829 Human
    • Unigene: 606106 Human
    • Unigene: 257437 Mouse
    • Unigene: 23200 Rat
    see all
  • Alternative names

    • CADH2_HUMAN antibody
    • Cadherin 2 antibody
    • Cadherin 2 N cadherin neuronal antibody
    • Cadherin 2 type 1 antibody
    • Cadherin 2 type 1 N cadherin neuronal antibody
    • Cadherin 2, type 1, N-cadherin (neuronal) antibody
    • Cadherin-2 antibody
    • Cadherin2 antibody
    • Calcium dependent adhesion protein neuronal antibody
    • CD325 antibody
    • CD325 antigen antibody
    • CDH2 antibody
    • CDHN antibody
    • CDw325 antibody
    • CDw325 antigen antibody
    • N cadherin 1 antibody
    • N-cadherin antibody
    • NCAD antibody
    • Neural cadherin antibody
    • OTTHUMP00000066304 antibody
    • OTTHUMP00000067378 antibody
    see all

Images

  • Western blot - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)
    Western blot - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)
    All lanes : Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate at 10 µg
    Lane 2 : Brain (Mouse) Tissue Lysate at 10 µg
    Lane 3 : Brain (Human) Tissue Lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 100 kDa
    Observed band size: 125 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab18203 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    The N Cadherin protein has a predicted molecular weight of 100 kDa, however it is extensively glycosylated and has been shown to run in the 125-135 kDa region (SwissProt data).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)Image courtesy of Mr Carl Hobbs, Kings College London.

    Anti N-cadherin (ab18203) staining of mouse brain using immunohistochemistry (formaldehyde-fixed, paraffin-embedded sections). Heat-mediated antigen retrieval was carried out using citric acid. Samples were incubated with primary antibody (1/1000) for two hours at room temperature. A biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)Image courtesy of Mr Carl Hobbs, Kings College London.

    Anti N-cadherin (ab18203) staining of human ovarian cancer tissue using immunohistochemistry (formaldehyde-fixed, paraffin-embedded sections). Heat-mediated antigen retrieval was carried out using citric acid. Samples were incubated with primary antibody (1/1000) for two hours at room temperature. A biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)Image courtesy of Mr Carl Hobbs, Kings College London.

    Anti N-cadherin (ab18203) staining in a human melanoma xenograft mouse model using immunohistochemistry (formaldehyde-fixed, paraffin-embedded sections). Heat-mediated antigen retrieval was carried out using citric acid. Samples were incubated with primary antibody (1/1000) for two hours at room temperature. A biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)Image courtesy of Mr Carl Hobbs, Kings College London.

    Anti N-cadherin (ab18203) staining of E17 developing rat retina using immunohistochemistry (formaldehyde-fixed, paraffin-embedded sections). Heat-mediated antigen retrieval was carried out using citric acid. Samples were incubated with primary antibody (1/1000) for two hours at room temperature. A biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)

    IHC image of N Cadherin staining in Human liver cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18203, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)
    Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)

    Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203) stained human embryonic stem cells differentiated into mesoderm.

  • Immunoprecipitation - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)
    Immunoprecipitation - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)
    N Cadherin was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to N Cadherin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab18203.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 135kDa: N Cadherin
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)This image is courtesy of an anonymous Abreview
    ab18203 staining N Cadherin in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a 10 mM citrate buffer pH6.0. Samples were incubated with primary antibody (1/100 in PBS plus casein) for 90 minutes at 37°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)
    Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)This image is courtesy of an Abreview submitted by Dr. Ann Wheeler.

    Paraformaldehyde-fixed, 0.2% Triton X100 permeabilized HaCaT (human keratinocyte cell line) cells stained for N Cadherin (green) using ab18203 at 1/200 dilution in ICC/IF, followed by Donkey anti Rabbit Alexa Fluor 568 at 1/500 dilution.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203)

    Immunohistochemistry of kidney carcinoma staining N Cadherin with ab18203 at 1μg/ml.

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (327)

    Publishing research using ab18203? Please let us know so that we can cite the reference in this datasheet.

    ab18203 has been referenced in 327 publications.

    • Zhao W  et al. Splicing factor derived circular RNA circUHRF1 accelerates oral squamous cell carcinoma tumorigenesis via feedback loop. Cell Death Differ 27:919-933 (2020). PubMed: 31570856
    • Xu G  et al. Up-regulated microRNA-33b inhibits epithelial-mesenchymal transition in gallbladder cancer through down-regulating CROCC. Biosci Rep 40:N/A (2020). PubMed: 31799620
    • Sun X  et al. Effect of Loureirin B on Crohn's disease rat model induced by TNBS via IL-6/STAT3/NF-?B signaling pathway. Chin Med 15:2 (2020). PubMed: 31911815
    • Li Q  et al. Silencing of synaptotagmin 13 inhibits tumor growth through suppressing proliferation and promoting apoptosis of colorectal cancer cells. Int J Mol Med 45:234-244 (2020). PubMed: 31939613
    • Ning JZ  et al. MiR-425 Promotes Migration and Invasion in Bladder Cancer by Targeting Dickkopf 3. J Cancer 11:3424-3432 (2020). PubMed: 32284738
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 53 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-N Cadherin antibody - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Zebrafish Tissue sections (Embryo)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 100x Citrate Buffer pH 6.0 (ab64236)
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Embryo
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 29 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-N Cadherin antibody - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 100x Citrate Buffer pH 6.0 (ab64236)
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 29 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-N Cadherin antibody - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 100x Citrate Buffer pH 6.0 (ab64236)
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 29 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-N Cadherin antibody - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Pig Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 100x Citrate Buffer pH 6.0 (ab64236)
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 23 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-N Cadherin antibody - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Liver
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 05 2020

    Western blot abreview for Anti-N Cadherin antibody - Intercellular Junction Marker

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    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (endothelial)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    endothelial
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Dr. Dhanush Haspula

    Verified customer

    Submitted May 12 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-N Cadherin antibody - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Liver
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 06 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-N Cadherin antibody - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Liver
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 06 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-N Cadherin antibody - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Kidney H9 Organoid)
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Kidney H9 Organoid
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 06 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-N Cadherin antibody - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 06 2020

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