Overview

  • Product name
    Anti-N Cadherin antibody [SP90] - BSA and Azide free
    See all N Cadherin primary antibodies
  • Description
    Rabbit monoclonal [SP90] to N Cadherin - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide within Human N Cadherin aa 800-900 (C terminal). The exact sequence is proprietary.
    Database link: P19022

    Synthetic peptide within Human N Cadherin aa 800-900 (C terminal). The exact sequence is proprietary.
    Database link: P19022

  • Positive control
    • IHC-P: Human mesothelioma tissue FC: HeLa
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    ab240403 is a PBS-only buffer format of ab225719. Please refer to ab225719 for recommended dilutions, protocols, and image data.

     

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties

Applications

Our Abpromise guarantee covers the use of ab240403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Antigen retrieval: Boil tissue section in 1mM EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

 

Flow Cyt Use at an assay dependent concentration.

Target

  • Function
    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
  • Sequence similarities
    Contains 5 cadherin domains.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CADH2_HUMAN antibody
    • Cadherin 2 antibody
    • Cadherin 2 N cadherin neuronal antibody
    • Cadherin 2 type 1 antibody
    • Cadherin 2 type 1 N cadherin neuronal antibody
    • Cadherin 2, type 1, N-cadherin (neuronal) antibody
    • Cadherin-2 antibody
    • Cadherin2 antibody
    • Calcium dependent adhesion protein neuronal antibody
    • CD325 antibody
    • CD325 antigen antibody
    • CDH2 antibody
    • CDHN antibody
    • CDw325 antibody
    • CDw325 antigen antibody
    • N cadherin 1 antibody
    • N-cadherin antibody
    • NCAD antibody
    • Neural cadherin antibody
    • OTTHUMP00000066304 antibody
    • OTTHUMP00000067378 antibody
    see all

Images

  • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) labeling N Cadherin with purified ab225719 at 1/20 dilution (4.55 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225719).

  • Formalin-fixed, paraffin-embedded human stomach tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225719).

  • Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling N Cadherin with ab225719 at 1/100 (green) compared with an isotype control rabbit IgG (blue). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225719).

  • Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225719).

  • Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225719).

  • Formalin-fixed, paraffin-embedded human adrenal gland tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225719).

  • Formalin-fixed, paraffin-embedded human liver tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225719).

  • Formalin-fixed, paraffin-embedded human mesothelioma tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225719).

  • Formalin-fixed, paraffin-embedded human mesothelioma tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab225719).

     

References

ab240403 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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