Recombinant
RabMAb

Recombinant Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

Overview

  • Product name

    Anti-N Cadherin antibody [SP90] - C-terminal
    See all N Cadherin primary antibodies
  • Description

    Rabbit monoclonal [SP90] to N Cadherin - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide within Human N Cadherin aa 800-900 (C terminal). The exact sequence is proprietary.
    Database link: P19022

  • Positive control

    • IHC-P: Human mesothelioma, liver, adrenal gland fallopian tube, renal cell carcinoma, stomach, Human heart, Mouse liver, and Rat liver tissue; FC: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab225719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IHC-P 1/100.

Antigen retrieval: Boil tissue section in 1mM EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Target

  • Function

    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
  • Sequence similarities

    Contains 5 cadherin domains.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CADH2_HUMAN antibody
    • Cadherin 2 antibody
    • Cadherin 2 N cadherin neuronal antibody
    • Cadherin 2 type 1 antibody
    • Cadherin 2 type 1 N cadherin neuronal antibody
    • Cadherin 2, type 1, N-cadherin (neuronal) antibody
    • Cadherin-2 antibody
    • Cadherin2 antibody
    • Calcium dependent adhesion protein neuronal antibody
    • CD325 antibody
    • CD325 antigen antibody
    • CDH2 antibody
    • CDHN antibody
    • CDw325 antibody
    • CDw325 antigen antibody
    • N cadherin 1 antibody
    • N-cadherin antibody
    • NCAD antibody
    • Neural cadherin antibody
    • OTTHUMP00000066304 antibody
    • OTTHUMP00000067378 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling N Cadherin with Purified ab225719 at 1/100 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue sections labeling N Cadherin with Purified ab225719 at 1/100 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human heart tissue sections labeling N Cadherin with Purified ab225719 at 1/100 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling N Cadherin with Purified ab225719 at 1/100 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Flow cytometry analysis of Hela (human cervix adenocarcinoma) labeling N Cadherin with purified ab225719 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).

  • Formalin-fixed, paraffin-embedded human mesothelioma tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

  • Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling N Cadherin with ab225719 (green) compared with an isotype control rabbit IgG (blue). 

  • Formalin-fixed, paraffin-embedded human mesothelioma tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human liver tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human adrenal gland tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human stomach tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

References

ab225719 has not yet been referenced specifically in any publications.

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