Overview

  • Product name

    N Cadherin ELISA Kit
    See all N Cadherin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 9.6ng/ml 0.365 3.8%
    2 16 22.4ng/ml 0.963 4.3%
    3 16 38.2ng/ml 1.986 5.2%
    Inter-assay
    Sample n Mean SD CV%
    1 24 12.5ng/ml 0.675 5.4%
    2 24 26.4ng/ml 1.61 6.1%
    3 24 40.7ng/ml 2.768 6.8%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 20 pg/ml
  • Range

    156 pg/ml - 10000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The N Cadherin Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213756) is designed for the quantitative measurement of Human N-Cadherin in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for Cadherin-2/N-Cadherin has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: D160-A724) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cadherin-2/N Cadherin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Cadherin-2/N-Cadherin amount of sample captured in plate.

  • Notes

    N Cadherin, also known as Cadherin-2 (CDH2), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. N-Cadherin is expressed in the brain, skeletal and cardiac muscle. N Cadherin is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human Cadherin-2 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Human Cadherin-2 antibody 1 x 130µl
    Lyophilized recombinant Human Cadherin-2 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
  • Sequence similarities

    Contains 5 cadherin domains.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Alternative names

    • CADH2_HUMAN
    • Cadherin 2
    • Cadherin 2 N cadherin neuronal
    • Cadherin 2 type 1
    • Cadherin 2 type 1 N cadherin neuronal
    • Cadherin 2, type 1, N-cadherin (neuronal)
    • Cadherin-2
    • Cadherin2
    • Calcium dependent adhesion protein neuronal
    • CD325
    • CD325 antigen
    • CDH2
    • CDHN
    • CDw325
    • CDw325 antigen
    • N cadherin 1
    • N-cadherin
    • NCAD
    • Neural cadherin
    • OTTHUMP00000066304
    • OTTHUMP00000067378
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • N-Cadherin ELISA Kit (ab213756) Standard Curve.

Protocols

References

This product has been referenced in:

  • Deng J  et al. Long non-coding RNA OIP5-AS1 functions as an oncogene in lung adenocarcinoma through targeting miR-448/Bcl-2. Biomed Pharmacother 98:102-110 (2018). WB ; Human . Read more (PubMed: 29247949) »
  • Qu W  et al. MiR-552 promotes the proliferation, migration and EMT of hepatocellular carcinoma cells by inhibiting AJAP1 expression. J Cell Mol Med N/A:N/A (2018). Read more (PubMed: 30597727) »
See all 2 Publications for this product

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