Key features and details
- Rabbit polyclonal to N Cadherin (phospho Y820)
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-N Cadherin (phospho Y820) antibody
See all N Cadherin primary antibodies
DescriptionRabbit polyclonal to N Cadherin (phospho Y820)
Specificityab76059 detects a 130 kDa band corresponding to N cadherin in western blots of serum-starved human endothelial cells treated with pervanadate, but it is not detected in untreated cells.
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic phospho-peptide (coupled to carrier protein) corresponding to amino acids surrounding tyrosine 820 in human N cadherin.
- Human endothelial cells treated with pervanadate (1 mM) for 30 min.
General notesDo not aliquot this antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab76059 was cross-adsorbed to phospho-N Cadherin (Tyr-860) and unphosphorylated N cadherin (Tyr-820) peptides before affinity purification using phospho-N cadherin (Tyr-820) peptide.
Our Abpromise guarantee covers the use of ab76059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 100 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionCadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
Sequence similaritiesContains 5 cadherin domains.
Cellular localizationCell membrane.
- Information by UniProt
- CADH2_HUMAN antibody
- Cadherin 2 antibody
- Cadherin 2 N cadherin neuronal antibody
All lanes : Anti-N Cadherin (phospho Y820) antibody (ab76059) at 1/1000 dilution
Lane 1 : Human endothelial cells untreated
Lane 2 : Human endothelial cells treated with pervanadate (1 mM) for 30 min
Lane 3 : Human endothelial cells treated with pervanadate (1 mM) for 30 min with phospho-N-cadherin (Tyr-820) peptide
Lane 4 : Human endothelial cells treated with pervanadate (1 mM) for 30 min with phospho-N-cadherin (Tyr-860) peptide
Predicted band size: 100 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab76059 has not yet been referenced specifically in any publications.