Anti-N Cadherin (phospho Y860) antibody (ab119752)
Key features and details
- Rabbit polyclonal to N Cadherin (phospho Y860)
- Suitable for: WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-N Cadherin (phospho Y860) antibody
See all N Cadherin primary antibodies -
Description
Rabbit polyclonal to N Cadherin (phospho Y860) -
Host species
Rabbit -
Specificity
Immunogen sequence is conserved in Human E Cadherin (Y835), P Cadherin (Y782), R Cadherin (Y870) and has significant homology to VE Cadherin (Y733). -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human N Cadherin (phospho Y860).
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Positive control
- C2C12 and Human endothelial cells treated with pervanadate.
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General notes
Do not aliquot.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab119752 was cross-adsorbed to phospho-N-Cadherin (Tyr-820) and unphosphorylated N-cadherin (Tyr-860) peptides before affinity purification using phospho-N-cadherin (Tyr-860) peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 120-130 kDa (predicted molecular weight: 99 kDa).
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ICC |
1/200.
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Notes |
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WB
1/1000. Detects a band of approximately 120-130 kDa (predicted molecular weight: 99 kDa). |
ICC
1/200. |
Target
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Function
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. -
Sequence similarities
Contains 5 cadherin domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1000 Human
- Entrez Gene: 12558 Mouse
- Omim: 114020 Human
- SwissProt: P19022 Human
- SwissProt: P15116 Mouse
- Unigene: 464829 Human
- Unigene: 606106 Human
- Unigene: 257437 Mouse
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Alternative names
- CADH2_HUMAN antibody
- Cadherin 2 antibody
- Cadherin 2 N cadherin neuronal antibody
see all
Images
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ab119752 at 1/200 dilution staining N Cadherin (phospho Y860) in Mouse C2C12 cells, treated with pervanadate (right). The cells on the left were not treated with pervanadate prior to analysis.
Detection used an appropriate secondary antibody conjugated to Cy3. -
Lanes 1-2 : Anti-N Cadherin (Cytoplasmic) at 1/1000 dilution
Lanes 3-4 : Anti-N Cadherin
(phospho Y820) at 1/1000 dilution
Lanes 5-6 : Anti-N Cadherin (a.a. 853-864) at 1/1000 dilution
Lanes 7-8 : Anti-N Cadherin (phospho Y860) antibody (ab119752) at 1/1000 dilution
Lanes 1 & 5 & 7 : Human endothelial cells, untreated
Lanes 2 & 4 & 8 : Human endothelial cells, treated with pervanadate (1 mM) for 30 min
Lane 3 : Human endothelial cells, untreated
Lane 6 : Human endothelial cells treated with pervanadate (1 mM) for 30 min
Predicted band size: 99 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab119752 has been referenced in 2 publications.
- de Souza JM et al. mGluR5 regulates REST/NRSF signaling through N-cadherin/ß-catenin complex in Huntington's disease. Mol Brain 13:118 (2020). PubMed: 32859226
- Xiong Y et al. Precursor N-cadherin mediates glial cell line-derived neurotrophic factor-promoted human malignant glioma. Oncotarget 8:24902-24914 (2017). Human . PubMed: 28212546