Overview

  • Product name
    Anti-N-epsilon acetyl Lysine antibody [11A1]
  • Description
    Mouse monoclonal [11A1] to N-epsilon acetyl Lysine
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Species independent
  • General notes
    The ability of this antibody to fix complement is unknown.

Properties

Applications

Our Abpromise guarantee covers the use of ab409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000.
ELISA 1/5000 - 1/50000.
IHC-P 1/500 - 1/5000.

Images

  • Western Blot analysis showing ab409 staining N-epsilon acetyl Lysine on immunoprecipitated ER-resident proteins from control (non-transfected) H4 cells.

References

This product has been referenced in:
  • Peng Y  et al. Deficient import of acetyl-CoA into the ER lumen causes neurodegeneration and propensity to infections, inflammation, and cancer. J Neurosci 34:6772-89 (2014). Read more (PubMed: 24828632) »
  • Ding Y  et al. Biochemical Inhibition of the Acetyltransferases ATase1 and ATase2 Reduces ß-Secretase (BACE1) Levels and Aß Generation. J Biol Chem 287:8424-33 (2012). WB . Read more (PubMed: 22267734) »
See all 8 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Answer

The carboxybenzyloxy (CBZ) is a blocking group used to protect the primary amine during organic synthesis. We have used this derivative for the immunogen synthesis. There was no acetylated group on the immunogen.

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Question
Answer

The immunogen used to prepare this mouse monoclonal antibody was the KLH-lysine Nalpha-CBZ If you have any additional questions, please contact us again.

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Answer

Ab409 used to be sold in lyophilized form and is now sold as a liquid. I apologize for the mistake on the datasheet and it has since been updated. Would you like a replacement vial of ab409? Please let me know and I can have it sent to you next week.

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Answer

The suppier of ab409 was able to inform us that Ab409 was not tested on p53 and p65.

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Answer

We would suggest using BSA in TBST solution. Do not hesite to contact us for any further information.

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Answer

Yes, ab409 -- Mouse monoclonal (11A1) to N-epsilon acetyl lysine is suitable for use in detecting acetylated histones and other acetylated proteins. Suggested dilution for Western blot is 1/500 to 1/5000.

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Question
Answer

This antibody should recognise all acetylated histones.

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Answer

Both antibodies have been found to work well in WB. However, neither have been assessd in a supershift experiment, therefore we can't guarantee results.

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Answer

All the information we have for this antibody is on the datasheet and at present, this is the only antibody agaisnt acetyl-lysine that we currently have in our product range.

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Question
Answer

Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we offer a replacement or reimbursement. We do not routinely sell samples. We also offer a USD20 Amazon gift voucher to researchers who feedback information about our products.

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1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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