N-epsilon-(Carboxyethyl) Lysine (CEL) Assay Kit (ab238540)

Overview

  • Product name

    N-epsilon-(Carboxyethyl) Lysine (CEL) Assay Kit
  • Detection method

    Colorimetric
  • Sample type

    Serum, Plasma, Cell Lysate, Purified protein
  • Assay type

    Competitive
  • Range

    0.1 µg/ml - 25 µg/ml
  • Product overview

    N-epsilon-(Carboxyethyl) Lysine (CEL) Assay Kit (ab238540) is designed for the rapid detection and quantitation of CEL protein adducts.


    First, a CEL conjugate is coated on the ELISA plate. The unknown CEL protein samples or CEL-BSA standards are then added to the CEL conjugate preabsorbed plate. After a brief incubation the anti-CEL monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The content of CEL protein adducts in unknown samples is determined by comparison with the pre-determined CEL-BSA standard curve. Despite the structure similarity between CEL and CML, the anti-CEL specific antibody in this kit will not cross react with CML protein adducts.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 96 tests
    100X Conjugate Diluent 1 x 300µl
    10X Wash Buffer 1 x 100ml
    Protein Binding Strip Well Plate 1 unit
    Anti-CEL Antibody (1000X) 1 x 10µl
    Assay Diluent 1 x 50ml
    CEL Conjugate 1 x 20µl
    CEL-BSA Standard 1 x 30µl
    Secondary Antibody, HRP Conjugate (1000X) 1 x 20µl
    Stop Solution 1 x 12ml
    Substrate Solution 1 x 12ml

Images

  • Typical CEL ELISA results.

    This data is for reference only and should not be used to interpret actual results.

Protocols

References

ab238540 has not yet been referenced specifically in any publications.

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