N-epsilon-(Carboxymethyl) Lysine (CML) Assay Kit (ab238541)

Overview

  • Product name

    N-epsilon-(Carboxymethyl) Lysine (CML) Assay Kit
  • Detection method

    Colorimetric
  • Sample type

    Serum, Plasma, Cell Lysate, Purified protein
  • Assay type

    Competitive
  • Range

    0.05 µg/ml - 12.5 µg/ml
  • Product overview

    N-epsilon-(Carboxymethyl) Lysine (CML) Assay Kit (ab238541) specifically detects CML formation with a high level of sensitivity.


    First, a CML conjugate is coated on the ELISA plate. The unknown CML protein samples or CML-BSA standards are then added to the CML conjugate preabsorbed plate. After a brief incubation, the anti-CML monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The content of CML protein adducts in unknown samples is determined by comparison with the predetermined CML-BSA standard curve.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 96 tests 32 tests
    100X Conjugate Diluent 1 x 300µl 1 x 100µl
    10X Wash Buffer 1 x 100ml 1 x 30ml
    Protein Binding Strip Well Plate 1 unit 1 unit
    Anti-CML Antibody (1000X) 1 x 10µl 1 x 5µl
    Assay Diluent 1 x 50ml 1 x 20ml
    CML Conjugate 1 x 20µl 1 x 10µl
    CML-BSA Standard 1 x 50µl 1 x 20µl
    Secondary Antibody, HRP Conjugate (1000X) 1 x 20µl 1 x 20µl
    Stop Solution 1 x 12ml 1 x 4ml
    Substrate Solution 1 x 12ml 1 x 4ml

Images

  • Typical CML ELISA results.

    This data is for reference only and should not be used to interpret actual results.

Protocols

References

ab238541 has not yet been referenced specifically in any publications.

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