Anti-N epsilon gamma glutamyl Lysine antibody [81D4] (ab424)
Key features and details
- Mouse monoclonal [81D4] to N epsilon gamma glutamyl Lysine
- Suitable for: IHC-P, ELISA, IHC-Fr
- Reacts with: Species independent
- Isotype: IgM
Overview
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Product name
Anti-N epsilon gamma glutamyl Lysine antibody [81D4] -
Description
Mouse monoclonal [81D4] to N epsilon gamma glutamyl Lysine -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, ELISA, IHC-Frmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic isopeptide.
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General notes
The ability of this antibody to fix complement is unknown. The isopeptide may be purchased from Bachem Catalog number G-1970.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 2% Sucrose, 5.8% Sodium chloride, Phosphate Buffer -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
81D4 -
Myeloma
unknown -
Isotype
IgM -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/800.
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|
ELISA |
Use at an assay dependent concentration.
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|
IHC-Fr | (1) |
1/50 - 1/800.
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Notes |
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IHC-P
1/50 - 1/800. |
ELISA
Use at an assay dependent concentration. |
IHC-Fr
1/50 - 1/800. |
Images
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ab424 staining N epsilon gamma glutamyl Lysine in skin tissue sections.
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ab424 staining N epsilon gamma glutamyl Lysine in kidney tissue sections by Immunohistochemistry (Frozen sections). Sections A through D were stained for insoluble tissue transglutaminase, while E through H were stained for N epsilon gamma glutamyl Lysine crosslink. A, B, E, F were control kidneys; C, D, G, H were diseased kidneys showing increased staining in the expanded interstitium, the mesangium and periglomerular.
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab424 has been referenced in 17 publications.
- Yemanyi F et al. Crosslinked Extracellular Matrix Stiffens Human Trabecular Meshwork Cells Via Dysregulating ß-catenin and YAP/TAZ Signaling Pathways. Invest Ophthalmol Vis Sci 61:41 (2020). PubMed: 32832971
- Mižíková I et al. Targeting transglutaminase 2 partially restores extracellular matrix structure but not alveolar architecture in experimental bronchopulmonary dysplasia. FEBS J N/A:N/A (2018). PubMed: 29935061
- Raychaudhuri U et al. Tissue Transglutaminase Elevates Intraocular Pressure in Mice. Invest Ophthalmol Vis Sci 58:6197-6211 (2017). PubMed: 29222550
- Penumatsa KC et al. Transglutaminase 2 in pulmonary and cardiac tissue remodeling in experimental pulmonary hypertension. Am J Physiol Lung Cell Mol Physiol 313:L752-L762 (2017). PubMed: 28775095
- Sirikharin R et al. Role of astakine1 in regulating transglutaminase activity. Dev Comp Immunol 76:77-82 (2017). PubMed: 28528959