Anti-N-ethylmaleimide-sensitive fusion protein antibody (ab245595)
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- References
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Overview
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Product name
Anti-N-ethylmaleimide-sensitive fusion protein antibody
See all N-ethylmaleimide-sensitive fusion protein primary antibodies -
Description
Rabbit polyclonal to N-ethylmaleimide-sensitive fusion protein -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Orangutan
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Immunogen
Synthetic peptide within Human N-ethylmaleimide-sensitive fusion protein aa 500-550. The exact sequence is proprietary.
Database link: P46459 -
Positive control
- IP: HEK-293T whole cell lysate.
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General notes
ab245959 has not performed satisfactorily when used for WB of N-ethylmaleimide-sensitive fusion protein in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of N-ethylmaleimide-sensitive fusion protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245595 was affinity purified using an epitope specific to N-ethylmaleimide-sensitive fusion protein immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab245595 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. -
Sequence similarities
Belongs to the AAA ATPase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 4905 Human
- Entrez Gene: 18195 Mouse
- Entrez Gene: 60355 Rat
- Omim: 601633 Human
- SwissProt: P46459 Human
- SwissProt: P46460 Mouse
- SwissProt: Q9QUL6 Rat
- Unigene: 431279 Human
see all -
Alternative names
- N ethylmaleimide sensitive factor antibody
- N ethylmaleimide sensitive factor like protein antibody
- N ethylmaleimide sensitive fusion protein antibody
see all
Images
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Immunoprecipitation - Anti-N-ethylmaleimide-sensitive fusion protein antibody (ab245595)N-ethylmaleimide-sensitive fusion protein was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245595 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab245595 at 1 µg/ml.
Lane 1: ab245595 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
Lysates prepared in NETN buffer.
Datasheets and documents
References
ab245595 has not yet been referenced specifically in any publications.
