Product nameAnti-N-ethylmaleimide-sensitive fusion protein antibody [NSF-1]
See all N-ethylmaleimide-sensitive fusion protein primary antibodies
DescriptionMouse monoclonal [NSF-1] to N-ethylmaleimide-sensitive fusion protein
Tested applicationsSuitable for: ICC/IF, WB, Flow Cyt, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Cow, Human
Recombinant full length protein corresponding to Human N-ethylmaleimide-sensitive fusion protein.
- Rat PC12
Protein previously labeled as NSF.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.50
Constituents: 0.328% Sodium phosphate, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab16681 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||1/2000. Detects a band of approximately 76 kDa.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
FunctionRequired for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.
Sequence similaritiesBelongs to the AAA ATPase family.
- Information by UniProt
- N ethylmaleimide sensitive factor antibody
- N ethylmaleimide sensitive factor like protein antibody
- N ethylmaleimide sensitive fusion protein antibody
All lanes : Anti-N-ethylmaleimide-sensitive fusion protein antibody [NSF-1] (ab16681) at 0.5 µg/ml
Lane 1 : 3T3
Lane 2 : PC12
Lane 3 : MDBK
Lane 4 : Normal human fibroblasts
The diffuse bands at ~70 kDa were also present on a blot incubated with second antibody alone (data not shown).
ICC/IF image of ab16681 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16681, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Overlay histogram showing SH-SY5Y cells stained with ab16681 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16681, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This product has been referenced in:
- Sakano H et al. Proteomic analyses of nucleus laminaris identified candidate targets of the fragile X mental retardation protein. J Comp Neurol 525:3341-3359 (2017). Read more (PubMed: 28685837) »
- Chiang CF et al. Endocytic Pathways Used by Andes Virus to Enter Primary Human Lung Endothelial Cells. PLoS One 11:e0164768 (2016). WB ; Human . Read more (PubMed: 27780263) »