N-Formyl-Met-Leu-Phe, endogenous chemotactic peptide (ab141806)

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Overview

  • Product name

    N-Formyl-Met-Leu-Phe, endogenous chemotactic peptide

  • Description

    Potent endogenous chemotactic peptide

  • Biological description

    Potent endogenous chemotactic peptide and agonist for the formyl peptide receptor 1 (FPR1) (Ki = 38 nM). Chemoattractant for phagocytic leukocytes. Stimulates random and directed locomotion. Inducer of superoxide release. Active in vitro.

  • Purity

    > 98%

  • CAS Number

    59880-97-6

  • Chemical structure

    Chemical Structure

Properties

  • Molecular weight

    437.55

  • Molecular formula

    C21H31N3O5S

  • Sequence

    MLF (Modifications: N-terminal formyl)

  • PubChem identifier

    443295

  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.

  • Solubility overview

    Soluble in ethanol to 5 mM and in DMSO to 100 mM

  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    CC(C)CC(C(=O)NC(CC1=CC=CC=C1)C(=O)O)NC(=O)C(CCSC)NC=O

  • Source

    Synthetic

  • Research areas

Images

  • Functional Studies - N-Formyl-Met-Leu-Phe, endogenous chemotactic peptide (ab141806)
    Functional Studies - N-Formyl-Met-Leu-Phe, endogenous chemotactic peptide (ab141806)
    N-Formyl-Met-Leu-Phe activates Ca2+ transients in differentiated HL-60 cells.

    Cells were loaded with Fluo-3 AM (ab145254). Changes in intracellular Ca2+ were detected via changes in Fluo-3 emission following application (indicated by arrow) of 1 µM N-Formyl-Met-Leu-Phe (ab141806), (green) compared to control (black, saline perfusion).

References

This product has been referenced in:

  • Mills JS  et al. Characterization of the binding site on the formyl peptide receptor using three receptor mutants and analogs of Met-Leu-Phe and Met-Met-Trp-Leu-Leu. J Biol Chem 275:39012-7 (2000). Read more (PubMed: 10960471) »
  • Le Y  et al. Novel pathophysiological role of classical chemotactic peptide receptors and their communications with chemokine receptors. Immunol Rev 177:185-94 (2000). Read more (PubMed: 11138775) »
  • Katada T  et al. Synergistic activation of a family of phosphoinositide 3-kinase via G-protein coupled and tyrosine kinase-related receptors. Chem Phys Lipids 98:79-86 (1999). Read more (PubMed: 10358930) »
  • Showell HJ  et al. The structure-activity relations of synthetic peptides as chemotactic factors and inducers of lysosomal secretion for neutrophils. J Exp Med 143:1154-69 (1976). Read more (PubMed: 1262785) »
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