N-Formyl-Met-Leu-Phe, endogenous chemotactic peptide (ab141806)
- Datasheet
- References
Overview
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Product name
N-Formyl-Met-Leu-Phe, endogenous chemotactic peptide -
Description
Potent endogenous chemotactic peptide -
Biological description
Potent endogenous chemotactic peptide and agonist for the formyl peptide receptor 1 (FPR1) (Ki = 38 nM). Chemoattractant for phagocytic leukocytes. Stimulates random and directed locomotion. Inducer of superoxide release. Active in vitro. -
Purity
> 98% -
CAS Number
59880-97-6 -
Chemical structure
Properties
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Molecular weight
437.55 -
Molecular formula
C21H31N3O5S -
Sequence
MLF (Modifications: N-terminal formyl) -
PubChem identifier
443295 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in ethanol to 5 mM and in DMSO to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC(C)CC(C(=O)NC(CC1=CC=CC=C1)C(=O)O)NC(=O)C(CCSC)NC=O -
Source
Synthetic
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Research areas
Images
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Functional Studies - N-Formyl-Met-Leu-Phe, endogenous chemotactic peptide (ab141806)N-Formyl-Met-Leu-Phe activates Ca2+ transients in differentiated HL-60 cells.Cells were loaded with Fluo-3 AM (ab145254). Changes in intracellular Ca2+ were detected via changes in Fluo-3 emission following application (indicated by arrow) of 1 µM N-Formyl-Met-Leu-Phe (ab141806), (green) compared to control (black, saline perfusion).
References
This product has been referenced in:
- Mills JS et al. Characterization of the binding site on the formyl peptide receptor using three receptor mutants and analogs of Met-Leu-Phe and Met-Met-Trp-Leu-Leu. J Biol Chem 275:39012-7 (2000). Read more (PubMed: 10960471) »
- Le Y et al. Novel pathophysiological role of classical chemotactic peptide receptors and their communications with chemokine receptors. Immunol Rev 177:185-94 (2000). Read more (PubMed: 11138775) »
- Katada T et al. Synergistic activation of a family of phosphoinositide 3-kinase via G-protein coupled and tyrosine kinase-related receptors. Chem Phys Lipids 98:79-86 (1999). Read more (PubMed: 10358930) »
- Showell HJ et al. The structure-activity relations of synthetic peptides as chemotactic factors and inducers of lysosomal secretion for neutrophils. J Exp Med 143:1154-69 (1976). Read more (PubMed: 1262785) »
