Recombinant Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18982-8R-3] to n-Myc/MYCN - ChIP Grade
- Suitable for: ChIP, WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade
See all n-Myc/MYCN primary antibodies -
Description
Rabbit monoclonal [EPR18982-8R-3] to n-Myc/MYCN - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: ChIP, WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: IMR-32 whole cell lysate. IP: IMR-32 whole cell lysate. ChIP: Chromatin prepared from IMR-32 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 49% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18982-8R-3 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ChIP |
Use 5 µg for 25 µg of chromatin.
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WB |
1/1000. Detects a band of approximately 49-62 kDa (predicted molecular weight: 50 kDa).
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IP |
1/30.
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Notes |
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ChIP
Use 5 µg for 25 µg of chromatin. |
WB
1/1000. Detects a band of approximately 49-62 kDa (predicted molecular weight: 50 kDa). |
IP
1/30. |
Target
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Function
May function as a transcription factor. -
Involvement in disease
Note=Amplification of the N-MYC gene is associated with a variety of human tumors, most frequently neuroblastoma, where the level of amplification appears to increase as the tumor progresses.
Defects in MYCN are the cause of microcephaly-oculo-digito-esophageal-duodenal syndrome (MODED) [MIM:164280]; also known as oculodigitoesophagoduodenal syndrome (ODED). Microcephaly-oculo-digito-esophageal-duodenal syndrome is characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability and limb malformations. Cardiac and renal malformations, vertebral anomalies, and deafness have also been described.
Defects in MYCN are the cause of microcephaly and digital abnormalities with normal intelligence (MCPHDANI) [MIM:602585]. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Developmental stage
Expressed during fetal development. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4613 Human
- Omim: 164840 Human
- SwissProt: P04198 Human
- Unigene: 25960 Human
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Alternative names
- bHLHe37 antibody
- Class E basic helix-loop-helix protein 37 antibody
- MODED antibody
see all
Images
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Chromatin was prepared from IMR-32 cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25 µg of chromatin, 5 µg of ab227822 (red), and 20 µl of Protein A/G sepharose beads. 5 μg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (SYBR green approach). Primers and probes are located in the first kb of the transcribed region.
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All lanes : Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822) at 1/1000 dilution
Lane 1 : IMR-32 (human neuroblastoma neuroblast cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 50 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
Exposure time: 103 secondsBlocking and dilution buffer: 5% NFDM/TBST
The expression profile observed is consistent with what has been described in the literature (PMID: 11034201; PMID: 27197171; PMID: 23792191).
Negative control: HeLa (PMID: 27197171).
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n-Myc/MYCN was immunoprecipitated from 0.35 mg of IMR-32 (human neuroblastoma neuroblast cell line) whole cell lysate with ab227822 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227822 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: IMR-32 whole cell lysate 10 μg (Input).
Lane 2: ab227822 IP in IMR-32 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227822 in IMR-32 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.The expression profile observed is consistent with what has been described in the literature (PMID: 17938259; PMID: 2657399).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab227822 has been referenced in 1 publication.
- Qiu Q et al. A prognosis model for clear cell renal cell carcinoma based on four necroptosis-related genes. Front Med (Lausanne) 9:942991 (2022). PubMed: 36016998